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Fig. 2. Degeneration of Pten-deficient mouse RPE cannot be rescued by RA supplement. (A) Pten-cko and TRP1-Cre mice were injected with at-RA (20 mg/kg) intraperitoneally at every other day from P21 to P50. The cells expressing specific markers, including Otx2 (positive in RPE, photoreceptor, bipolar cell), Rhod (positive in rod photoreceptors), and Sox2 (positive in MG and amacrine cell subsets), in P50 Pten-cko and TRP1-Cre mice eye sections were examined by immunohistochemistry (see details in Materials and Methods section). GCL, ganglion cell layer. (B) DAPI-labeled nuclei of RPE, ONL and INL cells were counted and the relative numbers are shown in the graph (n = 5, 5 independent litters). Error bars denote SD. **P < 0.01, ***P < 0.001. (C) Polarity of mouse RPE was determined by immunostaining of β-catenin and ezrin, which are enriched in the basolateral and apical sides of the RPE, respectively.
Mol. Cells 2021;44:613~622
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