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Fig. 1. Decrease of retinoid metabolism enzymes in Pten-cko mouse RPE.
(A) Relative levels of mRNA of retinoid metabolism enzymes in P21 TRP1-Cre and Pten-cko littermate mouse RPE were examined by RT-PCR. Relative amounts of total mRNA and the loss of Pten gene expression in the samples used for the experiments were determined by RT-PCR of actinβ1 and of Pten, respectively. (B) RT-PCR band intensities were measured by Image-J software and the ratio to the intensity values of TRP1-Cre samples are shown in the graph (n = 3, 3 independent litters). (C) Relative levels of Adh1, Adh7, Rdh5, Rdh10, and Rpe65 proteins in TRP1-Cre and Pten-cko mouse RPE and retina were determined by WB. The concentration of ROL (D) and RA (E) in P21 TRP1-Cre and Pten-cko mouse RPE (20 mg tissue) and retina (90 mg tissue) were determined by ELISA (see details in Materials and Methods section). Error bars denote SD. n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001.
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