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Fig. 2. General schemes and designs of single-cell screening approaches. (A) General scheme of single-cell screening approaches. A library of gene perturbations is introduced into target cells as a pool and their effects are measured at a single-cell resolution to evaluate different molecular endpoints. (B) Design of cell engineering vectors to be detected by single-cell techniques. Gene perturbation targets are identified by incorporating the identifier sequences into transcripts that can be identified by single-cell techniques. (C) Single-cell lineage tracing methods. CRISPR scarring or barcode labeling techniques are used to generate diverse pseudo-genotypes in developing/differentiating cells, which can be detected at the RNA or DNA level in combination with single-cell transcriptome analyses. RFP, red fluorescent protein; GFP, green fluorescent protein.
Mol. Cells 2021;44:127~135
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