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Fig. 5. Cells that fail to form SGs die at the late stage of neuronal differentiation. (A) hBM-MSCs, hBM-MSC which are transfected with either wild type or mutant eIF2α and salubrinal treated hBM-MSCs were differentiated into neurons. Cells were observed at the indicated times. Scale bars = 100 μm. (B) Neuronally differentiated cells were counted (n = 4, *P < 0.05, **P < 0.005, Student’s t-test; ns, not significant). (C) Treated cells were incubated with induction media for the indicated times and the cell viability was measured (n = 3, *P < 0.05, **P < 0.005, Student’s t-test).
Mol. Cells 2020;43:848~855
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