Fig. 4. Disrupting SGs with mutant eIF2α cDNA interrupts neuronal differentiation. (A) hBM-MSCs were transfected with mutant eIF2α DNA (S51A and S51D) and incubated with neuronal induction media for the indicated times. Cells were immunostained for G3BP (orange) and NF-M (green). Scale bars = 50 μm. (B) S51A-transfected hBM-MSCs were incubated with induction media for the indicated times and ATF-4 protein was measured using specific antibody.

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