Fig. 3. Disrupting SG assembly using salubrinal impedes neuronal differentiation. (A) hBM-MSCs treated with/without salubrinal were induced to differentiate into neurons for the indicated times, and total protein was extracted. p-eIF2α and NF-M expression levels were measured with their specific antibodies. (B) Differentiating cells were fixed and stained with G3BP (orange) and TIA-1 (green) antibodies. SG-positive cells are indicated by an arrowheads. Scale bars = 50 μm. (C) Nascent proteins were measured using the AHA labeling in differentiating hBM-MSCs treated with/without salubrinal.

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