Fig. 2. Neuronal populations in the ARN and calcium oscillations in kisspeptin neurons in neonates and adults.
(A) t-distributed stochastic neighbor embedding plots of neuronal cell types in the ARN are labeled according to the expression of a specific marker, or combination of marker genes, and colored according to subclustering. Clusters with gray labels likely originated from regions surrounding the ARN. In addition to kisspeptin (Kiss1) neurons, proopiomelanocortin (Pomc) and agouti-related peptide (Agrp) neurons are identified by single cell RNA-seq (Campbell et al., 2017
). (B) Synchronized calcium oscillation in kisspeptin neurons from neonatal mice organotypic slice culture with a period of approximately 3 min possibly received developmental regulations and reached 9 min of calcium oscillation in adults shown by fiber photometry (modified from Clarkson et al., 2017
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