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Fig. 2. Myeloid differentiation of iPSC-derived CD34+CD45+ cells, genome-edited to express a truncated form (d715) of CSF3R and transduced with RUNX1-D171N lentiviral expression vector or empty vector (ev) control. Cells were cultured in suspension for a total of 9 days in medium supplemented with myeloid growth factors, i.e., a cocktail of IL3, SCF, GM-CSF and G-CSF for the first 4 days, followed by G-CSF as the single growth factor for the next 5 days.
Mol. Cells 2020;43:139~144
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