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Fig. 6. HeLa cells infected with shCTL or shOGT lentivirus were injected into nude mice. After 3 months, assessments were performed, and protein was extracted from lung tissue. (A) Lysates were analysed by western blotting for O-GlcNAc, OGT, p65, and CXCR4. (B) Lysates were subjected to sWGA-lectin-affinity purification, and the precipitates were analysed by western blot for O-GlcNAc and p65. The inhibitory monosaccharide GlcNAc was added during sWGA-lectin-affinity purification. Data represent the mean ± SEM of three independent experiments. ***P < 0.001, *P < 0.05.
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