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Fig. 5. (A) HeLa cells infected with shCTL or shOGT lentivirus were injected into nude mice. After 3 months, lung tissue samples from control subjects and OGT-knockdown mice were embedded in paraffin blocks and sectioned for staining. Left panel shows H&E staining, middle panel shows immunofluorescence analysis of KI67 and right panel shows HPV 18 E6/E7. (B) To confirm the relationship between OGT and p65, double immunofluorescence staining was performed, and merged images were obtained .DAPI fluorescence is shown as blue, OGT immunofluorescence is shown as red, and p65 immunofluorescence is shown as green. Merged images show the co-localization of NFκB p65 and OGT. (C) To confirm the relationship between OGT and CXCR4, double immunofluorescence staining was performed, and merged images were obtained. DAPI fluorescence is shown as blue, OGT immunofluorescence is shown as red, and CXCR4 immunofluorescence is shown as green. Merged images show the co-localization of p65 and OGT. Upper images are from lung tissues of control mice, and lower images are from lung tissue of OGT knockdown mice. Scale bar, 50 μm. Samples were analysed under a fluorescence microscope.
Mol. Cells 2017;40:476~484 https://doi.org/10.14348/molcells.2017.2309
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