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Fig. 4. HeLa cells were treated with or without thiamet G and subjected to double immunostaining. (A) Double immunofluorescence analysis was performed for OGT and p65. DAPI fluorescence is shown as blue, OGT immunofluorescence is shown as green, and p65 immunofluorescence is shown as red. Merged images show the co-localization of OGT and p65. (B) Double immunofluorescence analysis of p65 and O-GlcNAc is shown. DAPI fluorescence is shown in blue, p65 immunofluorescence is shown in green, and O-GlcNAc immunofluorescence is shown as red. Merged images show the co-localization of p65 and O-GlcNAc. (C) Double immunofluorescence analysis of CXCR4 and p65 is shown. DAPI fluorescence is shown as blue, CXCR4 immunofluorescence is shown as green, and p65 immunofluorescence is shown as red. Merged images show the co-localization of CXCR4 and p65. Cells were treated with 10 μM of thiamet G for 24 h. Scale bar, 20 μm. Cells were analysed under a fluorescence microscope.
Mol. Cells 2017;40:476~484 https://doi.org/10.14348/molcells.2017.2309
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