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Fig. 2. (A) HeLa cells infected with either scramble shRNA (shCTL) or shRNA against OGT (shOGT) lentivirus were lysed and subjected to immunoblotting for O-GlcNAc, OGT, NFκB p65, and CXCR4. (B) HeLa cells were treated with or without DON or thiamet G for 24 h. Cells were lysed and analysed by western blotting for O-GlcNAc, NFκB p65, and CXCR4. Data represent mean ± SEM of three independent experiments. ***P < 0.001, **P < 0.005, *P < 0.05.
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