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Fig. 2. Schematic diagrams of the TRICK and Suntag (or FLAG)-MBS systems. (A) Schematic of the TRICK system. Both PCP-GFP and MCP-RFP are bound to the untranslated mRNA. During translation, ribosomes read through the PBS sequence and knock off PCP-GFP from the mRNA. After the first round of translation, the mRNA is only labeled with RFP. (B) Suntag (or FLAG)-MBS system. Translation of RFP-labeled mRNA can be monitored by the fluorescence signal from scFV-GFP clustered on the nascent polypeptides of Suntag (or FLAG).
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