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Fig. 2. Schematic diagram for stepwise neural differentiation of hESCs (H9), and their representative morphologies at each stage. Upper panels (A, C, E, and G) show low magnification, with enlarged images in the bottom panels (B, D, F, and H), respectively. (A, B) Rosette-like structures formed by co-culturing hESCs with PA6 stromal cells. (C, D) Neurospheres formed in suspension culture. (E, F) Dissociated neural precursor cells maintained on PLO/FN double-coated dishes. (G, H) Neurospheres differentiated Adapted from: Kim et al. (2012) Ann Biomed Eng 40:1268-76 into mature neurons in N2 medium containing BDNF. Abbreviations: hESCs, human embryonic stem cells; PLO/FN, poly-L-ornithine and fibronectin; BDNF, brain-derived neurotrophic factor.
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