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Fig. 1. The effect of the global competition between exo- and endo-miRNAs on derepression of endo-miRNA targets. (A) Among mRNAs without exo-miRNA 6?8mer sites, mRNAs with endo-miRNA 7-8mer sites (red) are significantly upregulated than those without endo-miRNA 6-8mer sites (blue). To count the number of endo-miRNA TSs, top 10 highly expressed endo-miRNAs in HeLa cell were considered (). (B) Correlation between 3′-UTR length and the number of endo-miRNA 7-8mer sites. Top 10 (red), 20 (green), and 30 (magenta) highly expressed endo-miRNAs were considered to measure the number of endo-miRNA 7-8mer sites in each case. (C) Using all the mRNAs included in our 74 microarray data, fold-change values of mRNAs in response to exo-miRNAs or siRNAs were correlated with the number of endo-miRNA 7-8mer sites. (D) Fold-change values were correlated with 3′-UTR length. Otherwise as in (C). (E, F) For a subset of mRNAs without exo-miRNA 6?8mer sites, the same correlation analyses as in (C, D) were repeated. (G) For mRNAs without exo-miRNA 6?8mer sites (blue), the correlation between the mRNA fold-change and the 3′-UTR length on a log scale was analyzed (thick lines) and the simple linear regression lines are depicted (thin lines). After discarding mRNAs with 6?8mer sites in the 3′-UTR for the top 10 (red), 20 (green), and 30 (magenta) highly expressed endo-miRNAs, the same correlation analysis was conducted. Each point represents mean fold-change and the log scaled 3′-UTR length of 15,000 mRNAs.
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