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Fig. 4. SIFR knockdown in particular subset neurons of the PI decreases sleep. (A) SIFR was depleted in various regions of fly brain, including the mushroom body (MB), fan-shaped body (FB), antennal lobe (AL), optic lobe (OL), pars intercerebralis (PI), and clock neurons. Neuron-specific effects of SIFR depletion on baseline sleep were monitored as in . Sleep change percentages were calculated as compared to the averaged total sleep time in UAS-SIFR RNAi/+ (black bars) or Gal4/+ (gray bars) control flies. (B, C) Total sleep time and sleep profile when SIFR expression was depleted using the 121y-Gal4 driver. (D, E) Total sleep time and sleep profile when SIFR expression was depleted using the c767-Gal4 driver. (F) Expression patterns of 121y-Gal4 (top) and c767-Gal4 (bottom) drivers were visualized by GFP expression (green) in adult fly brains. Enlarged PI regions containing SIFa-expressing PI neurons (red) are shown. Error bars indicate SEM. One-way ANOVA followed by Bonferroni’s Multiple Comparison test was used for statistical analysis. **p < 0.01; ***p < 0.001.
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