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Fig. 3. Pan-neuronal depletion of SIFR causes short sleep. (A) Total RNA was purified from adult fly heads. SIFR mRNA levels were quantified using real-time PCR in triplicate and normalized to Rp49 mRNA levels. Data show the average from three independent experiments. (B-F) SIFR RNAi transgene was expressed with a pan-neuronal Elav-Gal4 driver. Locomotor activities were recorded from individual female flies in LD cycles as in . Total sleep time (B, F), sleep profile (C), activity level during wake (D), and sleep bout duration (E) were calculated and averaged per each genotype. Error bars indicate SEM. Unpaired Student’s t-tests was used for statistical analysis when test was compared to one control. One-way ANOVA followed by Bonferroni’s Multiple Comparison test was used for statistical analysis when tests were compared to two controls. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.
Mol. Cells 2014;37:295~301
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