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Fig. 2. Pan-neuronal depletion of SIFa neuro-peptide mimics the ablation of SIFa-expressing neurons to decrease sleep. The SIFa RNAi transgene was expressed with a pan-neuronal Elav-Gal4 driver. Locomotor activities were recorded from individual female flies in LD cycles as in . Total sleep time (A), sleep profile (B), activity level during wake (C), and sleep bout duration (D) were calculated and averaged per each genotype. Error bars indicate SEM. One-way ANOVA followed by Bonferroni’s Multiple Comparison test was used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001.
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