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Fig. 1. Ablation of SIFa-expressing PI neurons shortens baseline sleep. (A) SIFa peptide diminishes in SIFa-expressing PI neurons when the apoptosis-inducing gene, head involution defective (hid) was ectopically expressed by SIFa-Gal4. Adult fly brains were dissected and labeled with an anti-SIFa antibody and FITC-conjugated secondary antibody (green). PI, pars intercerebralis; FB, fan-shaped body. (B-I) Baseline sleep is shortened and fragmented in both male and female flies with the ablation of SIFa-expressing PI neurons. Locomotor activities from individual flies were recorded in 12:12-h LD or DD cycles. Sleep profile (B, I), total sleep time (C, G, and H), activity level during wake (D), sleep bout duration (E), and sleep bout number (F) were calculated and averaged per each genotype. Error bars indicate standard error of mean (SEM). One-way analysis of variance (ANOVA) followed by Bonferroni’s Multiple Comparison test was used for statistical analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.
Mol. Cells 2014;37:295~301
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