Sieun S. Kim and Seung-Jae V. LeeMol. Cells 2019; 42(5): 379-385 https://doi.org/10.14348/molcells.2019.0077
Abstract : Non-coding RNAs (ncRNAs) comprise various RNA species, including small ncRNAs and long ncRNAs (lncRNAs). ncRNAs regulate various cellular processes, including transcription and translation of target messenger RNAs. Recent studies also indicate that ncRNAs affect organismal aging and conversely aging influences ncRNA levels. In this review, we discuss our current understanding of the roles of ncRNAs in aging and longevity, focusing on recent advances using the roundworm
Kyung Jin Lee, Deokhee Kang, and Hee-Sung ParkMol. Cells 2019; 42(5): 386-396 https://doi.org/10.14348/molcells.2019.0078
Abstract : Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.
Jian Du, Si-Tong Yang, Jia Liu, Ke-Xin Zhang, and Ji-Yan LengMol. Cells 2019; 42(5): 397-405 https://doi.org/10.14348/molcells.2018.0180
Abstract : The regulatory role of long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (
Richa Pasriga, Jinmi Yoon, Lae-Hyeon Cho, and Gynheung AnMol. Cells 2019; 42(5): 406-417 https://doi.org/10.14348/molcells.2019.0009
Abstract : RICE FLOWERING LOCUS T 1 (RFT1) is a major florigen that functions to induce reproductive development in the shoot apical meristem (SAM). To further our study of
Soomin Lee, Jeong-Ah Kim, Hee-Dae Kim, Sooyoung Chung, Kyungjin Kim, and Han Kyoung ChoeMol. Cells 2019; 42(5): 418-425 https://doi.org/10.14348/molcells.2019.2427
Abstract : Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.
Wonkyong Kim, Jee Soo Choi, Daun Kim, Doohang Shin, Shinae Suk, and Younghoon LeeMol. Cells 2019; 42(5): 426-439 https://doi.org/10.14348/molcells.2019.0040
Abstract : Many small RNAs (sRNAs) regulate gene expression by base pairing to their target messenger RNAs (mRNAs) with the help of Hfq in