Directed Differentiation of Pluripotent Stem Cells by Transcription Factors
Abstract
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have been used as promising tools for regenerative medicine, disease modeling, and drug screening. Traditional and common strategies for pluripotent stem cell (PSC) differentiation toward disease-relevant cell types depend on sequential treatment of signaling molecules identified based on knowledge of developmental biology. However, these strategies suffer from low purity, inefficiency, and time-consuming culture conditions. A growing body of recent research has shown efficient cell fate reprogramming by forced expression of single or multiple transcription factors. Here, we review transcription factor-directed differentiation methods of PSCs toward neural, muscle, liver, and pancreatic endocrine cells. Potential applications and limitations are also discussed in order to establish future directions of this technique for therapeutic purposes.
INTRODUCTION
Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold great promise in many biomedical fields. Indefinite proliferation of PSCs provides an unlimited cell source. Pluripotent differentiation potential enables PSCs to generate any desired cell type in vitro. PSC-derived cells such as neurons are particularly valuable for human due to the lack of alternative sources. Therefore, generation of functional and fully differentiated cells from PSCs has been an active field of stem cell research. Common strategies rely on a vast knowledge of developmental biology learned from animal studies. Small molecules and recombinant signaling proteins have been applied to PSC differentiation in order to derive specific lineage cells. Despite the attempt to faithfully mimic in vivo development, these approaches suffer from several major limitations. The first limitation is that procedures are time-consuming. Neural differentiation, for example, usually takes 4~6 weeks and functional maturation requires a few more months depending on neuronal subtypes (Tao and Zhang, 2016; Xie et al., 2013). The second limitation is variability with low purity. Dramatic difference in differentiation potentials across particular PSC lines has been well documented in recent studies (Bock et al., 2011; Hu et al., 2010; Kajiwara et al., 2012; Kim et al., 2011; Koyanagi-Aoi et al., 2013; Osafune et al., 2008; Wu et al., 2007). This variability influences the response of PSCs to signaling molecules, resulting in different purity of desired cell types. Furthermore, difficulty in quality control of small molecules and recombinant proteins adds more variability to protocols. Given that large scale production of mature cells with high purity is necessary for clinical applications, alternative strategies might shed light on how to produce disease-relevant cell types from PSCs.
During embryonic development, cell fates are determined by a network of transcription factors. Consistently, successful reprogramming of cell fates by forced induction of single or a combination of transcription factors has been reported. Most strikingly, overexpression of four transcription factors (OCT4, SOX2, KLF4, c-MYC) can reprogram fibroblasts to PSCs (Takahashi et al., 2006). In some cases, single transcription factor is sufficient for direct reprogramming of fibroblasts to other cell types such as neurons and myoblasts (Chanda et al., 2014; Davis et al., 1987). These examples are very striking because cell fate acquisition relies on sequential requirement of multiple transcription factors over the course of development according to traditional views. To test the feasibility of transcription factor-directed PSC differentiation, systemic induction of transcription factors has been performed in mouse ESCs (mESCs)(Correa-Cerro et al., 2011; Nishiyama et al., 2009). Interestingly, 63 out of 137 transcription factors were able to initiate and direct specific differentiation programs when they were induced individually, suggesting that transcription factor induction could be an efficient strategy to direct PSC differentiation. In this review, we present PSC differentiation methods based on transcription factor induction. Potential limitations of this approach and possible solutions are also discussed.
NEURONS
Producing functionally mature neurons from human PSCs (hPSCs) is important to understand normal physiology of human neurons and pathology of neurological diseases. Conventional methods follow the developmental path of neuronal derivation. First, PSCs are differentiated into neural progenitor cells (NPCs) by embryoid body formation or dual SMAD inhibition (Chambers et al., 2009). After that, NPCs are directed into specific neurons by a combination of signaling molecules (Tabar and Studer, 2014; Tao and Zhang, 2016). Based on the finding that forced expression of three transcription factors (BRN2, ASCL1, and MYT1L) can reprogram mouse fibroblasts into functional neurons (Vierbuchen et al., 2010), whether the same combination of transcription factors could bypass the long developmental path from hPSCs to neurons has been tested (Pang et al., 2011). Surprisingly, hPSCs expressing these three transcription factors showed bipolar neuron-like morphologies as early as day 3. They expressed neuronal markers such as β-III-tubulin (also known as Tuj1) and MAP2 by day 8. Electrophysiological analysis showed that these hPSC-derived neurons generated action potentials as early as day 6. The rapid induction of neuronal fate implicates that transcription factor induction can bypass sequential developmental stages and drive direct differentiation of hPSCs into neurons. More strikingly, a single transcription factor, ASCL1, was sufficient to drive direct neuronal differentiation of both human and mouse PSCs (Chanda et al., 2014; Pang et al., 2011; Yamamizu et al., 2013). BRN2 and MYT1L likely contribute to neuronal maturation by increasing morphological complexity. ASCL1 induction during mESC differentiation increased the percentage of neural cells (PSA-NCAM+) from less than 5% (no ASCL1 induction) to almost 50%, highlighting the robustness of transcription factor-directed differentiation (Yamamizu et al., 2013). Other transcription factors previously known to play important roles in neurogenesis were also tested. Ectopic expression of NEUROG2 can efficiently drive mouse and human PSCs into pure excitatory neurons in less than two weeks (Thoma et al., 2012; Zhang et al., 2013). These NEUROG2-directed neurons showed neuronal transcription profiles, formed synapses, and produced robust action potentials. Furthermore, when these human neurons were transplanted into mouse brain, functional integration and active electrophysiological properties were observed (Zhang et al., 2013), suggesting potential applications of these neurons for cell replacement therapy. Forced induction of another proneural transcription factor, NEUROD1, also drove rapid differentiation of hPSCs into excitatory neurons (Zhang et al., 2013).
GABAergic interneurons are inhibitory neurons that balance neuronal excitation in the brain. Given that the majority of cortical interneurons are originated from the medial ganglionic eminence (MGE)(Wonders and Anderson, 2005), four transcription factors (ASCL1, DLX2, NKX2.1, and LHX6) have been chosen based on their expression in the MGE (Sun et al., 2016). Through extensive comparison, a combination of three transcription factors (ASCL1, DLX2, and LHX6) has been found to be able to drive hPSCs into GABA and MAP2 double-positive neurons within 10 days (Sun et al., 2016). Co-expression of miR-9/9* and miR-124 further increased the efficiency of interneuron derivation. These GABAergic interneurons exhibited electrophysiological properties within 6~8 weeks and functionally integrated into the host’s neural circuit upon transplantation to mouse brain (Sun et al., 2016). In another study, co-expression of ASCL1 and DLX2 was sufficient to differentiate hPSCs into GA-BAergic interneurons with ~90% efficiency (Yang et al., 2017). These interneurons showed electrophysiological properties at five weeks after transcription factor induction and survived well in the transplanted mouse brain. Interestingly, ASCL1 also contributed to the conversion of hPSCs into dopaminergic neurons when it was co-expressed with NURR1 and LMX1A (Theka et al., 2013). This combination of transcription factors produced mature and functional dopaminergic neurons within 21 days.
Motor neurons are typically produced from PSC-derived NPCs that are caudalized and ventralized by retinoic acid and sonic hedgehog, respectively (Li et al., 2005; Wichterle et al., 2002). These methods suffer from a long and inefficient differentiation process. To overcome such problem, motor neuron-specifying transcription factors have been identified based on temporal gene expression analysis upon retinoic acid/sonic hedgehog treatment (Hester et al., 2011). Forced expression of three transcription factors, NEUROG2, ISL1, and LHX3, in hPSC-derived NPCs accelerated motor neuron differentiation (Hester et al., 2011). At day 11 post transcription factor induction, cells expressed motor neuron markers HB9 and CHAT, formed neuromuscular junctions, and exhibited electrophysiological properties. Given that conventional methods using retinoic acid and sonic hedgehog take ~45 days to acquire mature motor neurons, transcription factor-directed motor neuron derivation could be a faster and more efficient alternative method. ISL1–LHX3 fusion proteins can also drive mESCs into mature motor neurons in the absence of sonic hedgehog by suppressing a spinal interneuron fate (Lee et al., 2012).
Many studies described above used a lentiviral vector for transcription factor delivery because this viral vector could infect PSCs in a highly efficient manner and drive stable expression of transgenes by host genome integration. However, host genome modifications could be a potential problem in terms of using transcription factor-directed differentiation methods in a clinical setting. To circumvent this issue, researchers have exploited alternative gene deliver tools such as protein transduction, synthetic mRNAs, and non-integrating viral vectors. For example, a cocktail of synthetic mRNAs encoding five transcription factors (NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2) induced rapid differentiation of hPSCs toward neurons (Goparaju et al., 2017). Synthetic mRNA-directed neurons showed electrophysiological properties as early as 7 days after mRNA transfection. A combination of synthetic mRNAs encoding ATOH1 and NEUROG2 was also tested to differentiate hiP-SCs into mature dopaminergic neurons (Xue et al., 2018). Delivery of three transcription factors, LHX3, NEUROG2, and ISL1, by non-integrating Sendai virus vectors efficiently drove hPSCs into HB9-positive and ChAT-positive motor neurons within two weeks after transduction (Goto et al., 2017). These examples suggest that transcription factor-directed differentiation methods can be further improved for clinical applications. The methods described above are summarized in Table 1.
PANCREATIC β-CELLS
β-cells are a type of endocrine cells in the pancreas that can produce insulins to control blood glucose levels. Because transplantation of β-cells is a promising approach to treat diabetes patients, many researchers have been working on generating functional β-cells from PSCs. Common and conventional methods for β-cell derivation follow the developmental process of pancreas (Van et al., 2009). PSCs are first induced into definitive endoderm which is further specified into pancreatic lineage cells. To facilitate β-cell differentiation, PAX4, an essential transcription factor for β-cell development, is overexpressed in mESCs (Blyszczuk et al., 2003). Constitutive expression of PAX4 increased the formation of insulin-producing cells from 10~20% to 60~80%. Furthermore, transplantation of PAX4-directed insulin-producing cells maintained normal blood glucose levels in streptozotocin-induced diabetic mice.
Other studies have also confirmed that forced induction of PAX4 could be an efficient method to derive insulin-producing β-cells from mouse and human PSCs (Liew et al., 2008; Lin et al., 2007). Other transcription factors that play key roles in pancreatic development have also been tested in PSCs. Forced expression of PDX1 could facilitate mESC differentiation toward insulin-producing cells although these cells lacked mature properties such as high insulin secretion and glucose-responsiveness (Miyazaki et al., 2004). It has been reported that biphasic induction of PDX1 that mimic the normal development of pancreatic β-cells had better effects on mouse and human ESC differentiation than constitutive expression (Bernardo et al., 2009). The ESC-derived β-cells secreted insulins and C-peptides. However, they failed to respond to glucose stimulation, suggesting that PDX1 alone might not be sufficient for mature β-cell derivation from PSCs. However, a later study reported that mESC-derived insulin-producing cells by PDX1 overexpression were glucose-responsive. They reduced hyperglycemia when they were transplanted into streptozotocin-induced diabetic mice (Raikwar and Zavazava, 2012). Such discrepancy could be due to different differentiation media conditions used to differentiate PDX1-expressing ESCs into insulin-producing cells. PDX1 induction has also been proven to be useful for β-cell derivation when it is combined with other transcription factors such as NKX6.1 and NEUROG3 (Ida et al., 2018; Kubo et al., 2011; Walczak et al., 2016). Moreover, it has been reported that induction of NKX2.2 alone can result in appearance of insulin-producing cells (stained by Dithizone) as early as 14 days in mESC-derived embryoid bodies although glucose-dependent secretion is not observed (Shiroi et al., 2005). Overall, forced induction of pancreatic transcription factors tested so far can drive a pancreatic β-cell fate from PSCs, although functional maturation might require other transcription factor networks.
Transcription factor-directed methods for pancreatic β-cell differentiation are summarized in Table 2.
SKELETAL AND CARDIAC MUSCLES
Muscular dystrophies including Duchenne muscular dystrophy and limb girdle muscular dystrophy are inherited disorders with skeletal muscle degeneration (Mercuri et al., 2013). PSC-derived skeletal muscles could be a promising source for transplantation therapy and disease modeling. Based on previous findings that MYOD1, a master transcription factor of myogenesis, can convert non-muscle cells into skeletal myocytes (Davis et al., 1987; Weintraub et al., 1989), whether MYOD1 induction could direct PSCs into skeletal muscles has been tested. MYOD1 overexpression facilitated myogenesis in mESC-derived embryoid bodies and generated skeletal muscle fibers (Dekel et al., 1992). However, only a subset of differentiating cells could be differentiated into skeletal muscles. Other studies have also confirmed that MYOD1 overexpression can induce myogenesis in mESCs (Ozasa et al., 2007; Yamamizu et al., 2013). Interestingly, MYOD1 is unable to induce the myogenic program in hESCs, although extensive myogenic conversion has been observed in human fibroblasts (Albini et al., 2013). However, co-expression of SWI/SNF component BAF60C (encoded by SMARCD3) and MYOD1 is capable of driving hESCs into muscle cells, suggesting that the presence of epigenetic barriers can block MYOD1 functions in hESCs (Albini et al., 2013). BAF60C2/MYOD1-directed myospheres showed spontaneous contraction as well as typical skeletal muscle markers (myogenin, MyH3, and MyH8) at 20 days after differentiation. Ectopic expression of the catalytic domain of histone demethylase JMJD3 also enabled MYOD1 to activate the myogenic program in hPSCs (Akiyama et al., 2016). However, another study has been reported that MYOD1 alone is sufficient to drive hiPSCs into skeletal muscles (Tanaka et al., 2013). Such difference could be attributed to different media conditions used for muscle differentiation or variability related to hESC and hiPSC lines. More studies are warranted in order to make a clear answer to this issue.
PAX3 is a key transcription factor that initiates myogenesis in early paraxial mesoderm. Consistently, forced expression of PAX3 in mESC-derived embryoid bodies can greatly promote myogenic differentiation (Darabi et al., 2008). Myo-genic progenitor cells can be further enriched from PAX3-directed cells by sorting out a PDGFαR+Flk1− population. Transplantation of PAX3-directed PDGFαR+Flk1− cells has shown potential for therapeutic engraftment in dystrophic mice (Darabi et al., 2008). Like PAX3, overexpression of PAX7 can also efficiently drive both human and mouse ESCs into muscle progenitor cells with muscle regeneration potential (Darabi et al., 2011, 2012).
To facilitate myocardial differentiation, transient overexpression of ISL1, a cardiac progenitor marker, has been tested in mESCs. Transfection of mESC-derived embryoid bodies with a ISL1-expressing construct increased the expression of cardiomyocyte markers (ACTC1, MLC2V, MYH7, SMA), suggesting an instructive role of ISL1 in myocardial differentiation (Kwon et al., 2009). The effect of ISL1 on myocardial differentiation has also been confirmed in hESCs by ISL1 protein transduction (Fonoudi et al., 2013). A combined delivery of three transcription factors, GATA4, MEF2C, and TBX5, to mESC-derived embryoid bodies activated the cardiac program and increased the efficiency of cardiomyocyte derivation up to 60% (Bai et al., 2015). Another combination of five transcription factors, GATA4, MEF2C, TBX5, ESRRG and MESP1, has been tested in hPSCs. Delivery of these transcription factors to hPSC-derived mesoderm strongly promoted cardiomyocyte differentiation (Jin et al., 2018). Furthermore, a single transcription factor, SHOX2, greatly facilitated the derivation of cardiac pacemaker cells when it was delivered into mESC-derived embryoid bodies (Ionta et al., 2015). Most SHOX2-directed embryoid bodies exhibited spontaneous beating. When they were transplanted to the left ventricular apex of rat heart, SHOX2-directed embryoid bodies functioned as biopacemakers. Thus, many attempts with single or a combination of transcription factors have been made to produce functional muscle cells from PSCs. However, it seems that transcription factor-directed differentiation methods are not yet mature enough to produce highly pure muscle cells with functional maturity in a short culture period. New combinations of transcription factors together with small-molecule epigenetic inhibitors should be tested to further improve PSC-derived muscle derivation in the future. Transcription factor-directed methods for muscle cell differentiation are summarized in Table 3.
HEPATOCYTES
Liver transplantation is a promising treatment for end-stage liver diseases. PSC-derived hepatocytes could not only be unlimited sources for cell therapy, but also serve as an experimental model for metabolic diseases. Several key transcription factors for liver development have been tested to develop directed PSC differentiation methods. HEX (also known as HHEX) is an essential transcription factor that functions at the early stage of hepatic development. Forced expression of HEX in mESC-derived endoderm can dramatically increase the expression of genes related to hepatocyte maturation and function (Kubo et al., 2010). These findings have also been confirmed in hPSCs (Inamura et al., 2011). hPSCs were differentiated into definitive endoderm by Activin A followed by adenoviral delivery of HEX. Transient overexpression of HEX was sufficient to promote hepatocyte derivation. HEX-directed cells expressed both α-fetoprotein (AFP) and albumin (ALB) on day 12. Furthermore, P450 cytochrome enzymes known to play a critical role in liver metabolism were also expressed in HEX-directed hepatoblasts (Inamura et al., 2011). Further maturation of hepatoblasts into functional hepatocytes was achieved by overexpression of HNF4α, a key regulator of liver-specific genes (Takayama et al., 2012a). Interestingly, forced induction of HNF4α in mESCs was able to bypass the natural order of hepatic differentiation and activate the hepatic program directly from mESCs (Yamamizu et al., 2013). ALB secretion and the uptake of low-density lipoproteins were observed in HNF4α-directed cells as early as 7 days after differentiation. In their study, other transcription factors, such as FOXA1, GATA2, and GATA3, could also directly drive mESCs into hepatocytes (Yamamizu et al., 2013).
Sequential delivery of transcription factors has also been tested for efficient PSC differentiation toward hepatocytes (Takayama et al., 2011; 2012b). Forced induction of either SOX17 or FOXA2 was used to differentiate hPSCs into definitive endoderm. SOX17-directed endoderm cells were driven to hepatocytes by HEX overexpression (Takayama et al., 2011). In FOXA2-directed endoderm cells, HNF1α transduction efficiently generated hepatocytes (Takayama et al., 2012b). Furthermore, a single-step protocol with a combination of transcription factors for hepatocyte derivation has been reported (Tomizawa et al., 2016). Forced induction of four transcription factors, CEBPA, CEBPB, FOXA1, and FOXA3, directly differentiated hiPSCs into hepatocytes when they were cultured in a medium that could promote hepatic differentiation. Transcription factor-directed methods for hepatocyte differentiation are summarized in Table 4.
CONCLUSIONS
During development, fully differentiated cells emerge through several intermediate stages. For example, ESCs first produce early neuroectoderm that gives rise to region-specific neural progenitor cells. Neural progenitor cells further differentiate into post-mitotic neurons, a type of fully differentiated cells in the brain. Even in this simplified description, the end-stage cell fate is derived through three sequential cell fate transitions. An outstanding question in developmental biology is if multiple transitions are necessary for the acquisition of fully differentiated cell fates. A growing body of recent evidence shows that cell fate acquisition/conversion can occur directly by forced expression of transcription factors, suggesting that multiple developmental steps are unnecessary for generating fully differentiated cells. In this review, we also described examples of direct conversion of PSCs into mature cell types by transcription factors. Overexpression of transcription factors related to late developmental stages could bypass early developmental processes and rapidly produce functionally mature cells from PSCs. This is especially prominent in neuronal differentiation because many studies have shown that single proneural transcription factor is sufficient to convert PSCs into functional neurons with almost 100% efficiency. However, transcription factor-directed PSC differentiation seems to be less effective in other lineages. Although transcription factor induction can promote lineage differentiation, protocols are not yet mature in terms of efficiency or functional maturity. In some cases, transcription factors failed to initiate the differentiation program in PSCs likely due to epigenetic barriers. Small-molecule epigenetic inhibitors could be helpful to reset PSCs to be responsive to late-stage transcription factors.
Despite major advantages of transcription factor-directed differentiation over conventional methods, current methods are not ready for clinical applications yet. Besides a few tests for functionality, extensive systemic analyses including transcriptome and epigenome should be performed to validate if transcription factor-directed cells are safe and mature as their in vivo counterparts. Furthermore, virus-mediated transcription factor delivery might be inappropriate for clinical settings. Alternative delivery methods such as protein transduction and synthetic RNA transfection are under active investigation. Given that simple and scalable differentiation procedures with high purity are major bottlenecks for therapeutic applications of PSC-derived cells, transcription factor-directed PSC differentiation methods could be used as a promising strategy for future cell therapy.
Article information
Articles from Mol. Cells are provided here courtesy of Mol. Cells
References
- Akiyama, T., Wakabayashi, S., Soma, A., Sato, S., Nakatake, Y., Oda, M., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., and Ko, S.B.H. (2016). Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells. Development. 143, 3674-3685.
- Albini, S., Coutinho, P., Malecova, B., Giordani, L., Savchenko, A., Forcales, S.V., and Puri, P.L. (2013). Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres. Cell Rep. 3, 661-670.
- Bai, F., Ho Lim, C., Jia, J., Santostefano, K., Simmons, C., Kasahara, H., Wu, W., Terada, N., and Jin, S. (2015). Directed differentiation of embryonic stem cells into cardiomyocytes by bacterial injection of defined transcription factors. Sci Rep. 5, 15014.
- Bernardo, A.S., Cho, C.H.H., Mason, S., Docherty, H.M., Pedersen, R.A., Vallier, L., and Docherty, K. (2009). Biphasic induction of Pdx1 in mouse and human embryonic stem cells can mimic development of pancreatic β-cells. Stem Cells. 27, 341-351.
- Blyszczuk, P., Czyz, J., Kania, G., Wagner, M., Roll, U., St-Onge, L., and Wobus, A.M. (2003). Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells. Proc Natl Acad Sci USA. 100, 998-1003.
- Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z.D., Ziller, M., Croft, G.F., Amoroso, M.W., and Oakley, D.H. (2011). Reference maps of human es and ips cell variation enable high-throughput characterization of pluripotent cell lines. Cell. 144, 439-452.
- Busskamp, V., Lewis, N.E., Guye, P., Ng, A.H., Shipman, S.L., Byrne, S.M., Sanjana, N.E., Murn, J., Li, Y., and Li, S. (2014). Rapid neurogenesis through transcriptional activation in human stem cells. Mol Syst Biol. 10, 760.
- Chambers, S.M., Fasano, C.A., Papapetrou, E.P., Tomishima, M., Sadelain, M., and Studer, L. (2009). Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 27, 275-280.
- Chanda, S., Ang, C.E., Davila, J., Pak, C., Mall, M., Lee, Q.Y., Ahlenius, H., Jung, S.W., Südhof, T.C., and Wernig, M. (2014). Generation of induced neuronal cells by the single reprogramming factor ASCL1. Stem Cell Reports. 3, 282-296.
- Correa-Cerro, L.S., Piao, Y., Sharov, A.A., Nishiyama, A., Cadet, J.S., Yu, H., Sharova, L.V., Xin, L., Hoang, H.G., and Thomas, M. (2011). Generation of mouse ES cell lines engineered for the forced induction of transcription factors. Sci Rep. 1, 167.
- Darabi, R., Gehlbach, K., Bachoo, R.M., Kamath, S., Osawa, M., Kamm, K.E., Kyba, M., and Perlingeiro, R.C.R. (2008). Functional skeletal muscle regeneration from differentiating embryonic stem cells. Nat Med. 14, 134-143.
- Darabi, R., Santos, F.N.C., Filareto, A., Pan, W., Koene, R., Rudnicki, M.A., Kyba, M., and Perlingeiro, R.C.R. (2011). Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors. Stem Cells. 29, 777-790.
- Darabi, R., Arpke, R.W., Irion, S., Dimos, J.T., Grskovic, M., Kyba, M., and Perlingeiro, R.C.R. (2012). Human ES- and iPS-derived myogenic progenitors restore DYSTROPHIN and improve contractility upon transplantation in dystrophic mice. Cell Stem Cell. 10, 610-619.
- Davis, R.L., Weintraub, H., and Lassar, A.B. (1987). Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 51, 987-1000.
- Dekel, I., Magal, Y., Pearson-White, S., Emerson, C.P., and Shani, M. (1992). Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene. New Biol. 4, 217-224.
- Fonoudi, H., Yeganeh, M., Fattahi, F., Ghazizadeh, Z., Rassouli, H., Alikhani, M., Mojarad, B.A., Baharvand, H., Salekdeh, G.H., and Aghdami, N. (2013). ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells. PLoS One. 8, e55577.
- Goparaju, S.K., Kohda, K., Ibata, K., Soma, A., Nakatake, Y., Akiyama, T., Wakabayashi, S., Matsushita, M., Sakota, M., and Kimura, H. (2017). Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. Sci Rep. 7, 42367.
- Goto, K., Imamura, K., Komatsu, K., Mitani, K., Aiba, K., Nakatsuji, N., Inoue, M., Kawata, A., Yamashita, H., and Takahashi, R. (2017). Simple derivation of spinal motor neurons from ESCs/iPSCs using sendai virus vectors. Mol Ther Methods Clin Dev. 4, 115-125.
- Hester, M.E., Murtha, M.J., Song, S., Rao, M., Miranda, C.J., Meyer, K., Tian, J., Boulting, G., Schaffer, D.V., and Zhu, M.X. (2011). Rapid and efficient generation of functional motor neurons from human pluripotent stem cells using gene delivered transcription factor codes. Mol Ther. 19, 1905-1912.
- Hu, B.Y., Weick, J.P., Yu, J., Ma, L.X., Zhang, X.Q., Thomson, J.A., and Zhang, S.C. (2010). Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc Natl Acad Sci. 107, 4335-4340.
- Ida, H., Akiyama, T., Ishiguro, K., Goparaju, S.K., Nakatake, Y., Chikazawa-nohtomi, N., Sato, S., Kimura, H., Yokoyama, Y., and Nagino, M. (2018). Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors. Stem Cell Res Ther. 9, 277.
- Inamura, M., Kawabata, K., Takayama, K., Tashiro, K., Sakurai, F., Katayama, K., Toyoda, M., Akutsu, H., Miyagawa, Y., and Okita, H. (2011). Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX. Mol Ther. 19, 400-407.
- Ionta, V., Liang, W., Kim, E.H., Rafie, R., Giacomello, A., Marbán, E., and Cho, H.C. (2015). SHOX2 overexpression favors differentiation of embryonic stem cells into cardiac pacemaker cells, improving biological pacing ability. Stem Cell Reports. 4, 129-142.
- Jin, Y., Liu, Y., Li, Z., Santostefano, K., Shi, J., Zhang, X., Wu, D., Cheng, Z., Wu, W., and Terada, N. (2018). Enhanced differentiation of human pluripotent stem cells into cardiomyocytes by bacteria-mediated transcription factors delivery. PLoS One. 13, e0194895.
- Kajiwara, M., Aoi, T., Okita, K., Takahashi, R., Inoue, H., Takayama, N., Endo, H., Eto, K., Toguchida, J., and Uemoto, S. (2012). Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells. Proc Natl Acad Sci USA. 109, 12538-12543.
- Kim, K., Zhao, R., Doi, A., Ng, K., Unternaehrer, J., Cahan, P., Hongguang, H., Loh, Y.H., Aryee, M.J., and Lensch, M.W. (2011). Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells. Nat Biotechnol. 29, 1117-1119.
- Koyanagi-Aoi, M., Ohnuki, M., Takahashi, K., Okita, K., Noma, H., Sawamura, Y., Teramoto, I., Narita, M., Sato, Y., and Ichisaka, T. (2013). Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells. Proc Natl Acad Sci USA. 110, 20569-20574.
- Kubo, A., Kim, Y.H., Irion, S., Kasuda, S., Takeuchi, M., Ohashi, K., Iwano, M., Dohi, Y., Saito, Y., and Snodgrass, R. (2010). The homeobox gene Hex regulates hepatocyte differentiation from embryonic stem cell-derived endoderm. Hepatology. 51, 633-641.
- Kubo, A., Stull, R., Takeuchi, M., Bonham, K., Gouon-Evans, V., Sho, M., Iwano, M., Saito, Y., Keller, G., and Snodgrass, R. (2011). Pdx1 and Ngn3 overexpression enhances pancreatic differentiation of mouse ES cell-derived endoderm population. PLoS One. 6, e24058.
- Kwon, C., Qian, L., Cheng, P., Nigam, V., Arnold, J., and Srivastava, D. (2009). A regulatory pathway involving Notch1/β-catenin/Isl1 determines cardiac progenitor cell fate. Nat Cell Biol. 11, 951-957.
- Lee, S., Cuvillier, J.M., Lee, B., Shen, R., Lee, J.W., and Lee, S.K. (2012). Fusion protein Isl1-Lhx3 specifies motor neuron fate by inducing motor neuron genes and concomitantly suppressing the interneuron programs. Proc Natl Acad Sci. 109, 3383-3388.
- Li, X.J., Du, Z.W., Zarnowska, E.D., Pankratz, M., Hansen, L.O., Pearce, R.A., and Zhang, S.C. (2005). Specification of motoneurons from human embryonic stem cells. Nat Biotechnol. 23, 215-221.
- Liew, C.G., Shah, N.N., Briston, S.J., Shepherd, R.M., Khoo, C.P., Dunne, M.J., Moore, H.D., Cosgrove, K.E., and Andrews, P.W. (2008). PAX4 enhances beta-cell differentiation of human embryonic stem cells. PLoS One. 3, e1783.
- Lin, H.T., Kao, C.L., Lee, K.H., Chang, Y.L., Chiou, S.H., Tsai, F.T., Tsai, T.H., Sheu, D.C., Ho, L.L.T., and Ku, H.H. (2007). Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method. World J Gastroenterol. 13, 1672-1679.
- Matsushita, M., Nakatake, Y., Arai, I., Ibata, K., Kohda, K., Goparaju, S.K., Murakami, M., Sakota, M., Chikazawa-Nohtomi, N., and Ko, S.B.H. (2017). Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors. Biochem Biophys Res Commun. 490, 296-301.
- Mercuri, E., Muntoni, F., Emery, A., Mercuri, E., Muntoni, F., Guglieri, M., Straub, V., Bushby, K., Lochmuller, H., and Bushby, K. (2013). Muscular dystrophies. Lancet (London, England). 381, 845-860.
- Miskinyte, G., Gr, M., Monni, E., Lam, M., Bengzon, J., Lindvall, O., Ahlenius, H., and Id, Z.K. (2018). Transcription factor programming of human ES cells generates functional neurons expressing both upper and deep layer cortical markers. PLoS One. 13, e0204688.
- Miyazaki, S., Yamato, E., and Miyazaki, J.I. (2004). Regulated expression of pdx-1 promotes in vitro differentiation of insulin-producing cells from embryonic stem cells. Diabetes. 53, 1030-1037.
- Nishiyama, A., Xin, L., Sharov, A.A., Thomas, M., Mowrer, G., Meyers, E., Piao, Y., Mehta, S., Yee, S., and Nakatake, Y. (2009). Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors. Cell Stem Cell. 5, 420-433.
- Osafune, K., Caron, L., Borowiak, M., Martinez, R.J., Fitz-Gerald, C.S., Sato, Y., Cowan, C.A., Chien, K.R., and Melton, D.A. (2008). Marked differences in differentiation propensity among human embryonic stem cell lines. Nat Biotechnol. 26, 313-315.
- Ozasa, S., Kimura, S., Ito, K., Ueno, H., Ikezawa, M., Matsukura, M., Yoshioka, K., Araki, K., and Yamamura Kich Abe, K. (2007). Efficient conversion of ES cells into myogenic lineage using the gene-inducible system. Biochem Biophys Res Commun. 357, 957-963.
- Pang, Z.P., Yang, N., Vierbuchen, T., Ostermeier, A., Fuentes, D.R., Yang, T.Q., Citri, A., Sebastiano, V., Marro, S., and Südhof, T.C. (2011). Induction of human neuronal cells by defined transcription factors. Nature. 476, 220-223.
- Raikwar, S.P., and Zavazava, N. (2012). PDX1-engineered embryonic stem cell-derived insulin producing cells regulate hyperglycemia in diabetic mice. Transplant Res. 9, 277.
- Shiroi, A., Ueda, S., Ouji, Y., Saito, K., Moriya, K., Sugie, Y., Fukui, H., Ishizaka, S., and Yoshikawa, M. (2005). Differentiation of embryonic stem cells into insulin-producing cells promoted by Nkx2.2 gene transfer. World J Gastroenterol. 11, 4161-4166.
- Sun, A.X., Yuan, Q., Tan, S., Xiao, Y., Wang, D., Khoo, A.T.T., Sani, L., Tran, H.D., Kim, P., and Chiew, Y.S. (2016). Direct induction and functional maturation of forebrain GABAergic neurons from human pluripotent stem cells. Cell Rep. 16, 1942-1953.
- Tabar, V., and Studer, L. (2014). Pluripotent stem cells in regenerative medicine: Challenges and recent progress. Nat Rev Genet. 15, 82-92.
- Takahashi, K., Yamanaka, S., Zhang, Y., Li, Y., Feng, C., Li, X., Lin, L., Guo, L., Wang, H., and Liu, C. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 126, 663-676.
- Takayama, K., Inamura, M., Kawabata, K., Tashiro, K., Katayama, K., Sakurai, F., Hayakawa, T., Furue, M.K., and Mizuguchi, H. (2011). Efficient and directive generation of two distinct endoderm lineages from human ESCs and iPSCs by differentiation stage-specific SOX17 transduction. PLoS One. 6, e21780.
- Takayama, K., Inamura, M., Kawabata, K., Katayama, K., Higuchi, M., Tashiro, K., Nonaka, A., Sakurai, F., Hayakawa, T., and Kusuda Furue, M. (2012a). Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4α transduction. Mol Ther. 20, 127-137.
- Takayama, K., Inamura, M., Kawabata, K., Sugawara, M., Kikuchi, K., Higuchi, M., Nagamoto, Y., Watanabe, H., Tashiro, K., and Sakurai, F. (2012b). Generation of metabolically functioning hepatocytes from human pluripotent stem cells by FOXA2 and HNF1α transduction. J Hepatol. 57, 628-636.
- Tanaka, A., Woltjen, K., Miyake, K., Hotta, A., Ikeya, M., Yamamoto, T., Nishino, T., Shoji, E., Sehara-Fujisawa, A., and Manabe, Y. (2013). Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling miyoshi myopathy in vitro. PLoS One. 8, e61540.
- Tao, Y., and Zhang, S.C. (2016). Neural subtype specification from human pluripotent stem cells. Cell Stem Cell. 19, 573-586.
- Theka, I., Caiazzo, M., Dvoretskova, E., Leo, D., Ungaro, F., Curreli, S., Managò, F., Dell’Anno, M.T., Pezzoli, G., and Gainetdinov, R.R. (2013). Rapid generation of functional dopaminergic neurons from human induced pluripotent stem cells through a single-step procedure using cell lineage transcription factors. Stem Cells Transl Med. 2, 473-479.
- Thoma, E.C., Wischmeyer, E., Offen, N., Maurus, K., Sirén, A.L., Schartl, M., and Wagner, T.U. (2012). Ectopic expression of neurogenin 2 alone is sufficient to induce differentiation of embryonic stem cells into mature neurons. PLoS One. 7, e38651.
- Tomizawa, M., Shinozaki, F., Motoyoshi, Y., Sugiyama, T., Yamamoto, S., and Ishige, N. (2016). Transcription factors and medium suitable for initiating the differentiation of human-induced pluripotent stem cells to the hepatocyte lineage. J Cell Biochem. 117, 2001-2009.
- Van, H.D., D’Amour, K.A., German, M.S., and Van Hoof, D. (2009). Derivation of insulin-producing cells from human embryonic stem cells. Stem Cell Res. 3, 73-87.
- Vierbuchen, T., Ostermeier, A., Pang, Z.P., Kokubu, Y., Südhof, T.C., and Wernig, M. (2010). Direct conversion of fibroblasts to functional neurons by defined factors. Nature. 463, 1035-1041.
- Walczak, M.P., Drozd, A.M., Stoczynska-Fidelus, E., Rieske, P., and Grzela, D.P. (2016). Directed differentiation of human iPSC into insulin producing cells is improved by induced expression of PDX1 and NKX6.1 factors in IPC progenitors. J Transl Med. 14, 341.
- Weintraub, H., Tapscott, S.J., Davis, R.L., Thayer, M.J., Adam, M.A., Lassar, A.B., and Miller, A.D. (1989). Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. Proc Natl Acad Sci. 86, 5434-5438.
- Wichterle, H., Lieberam, I., Porter, J.A., and Jessell, T.M. (2002). Directed differentiation of embryonic stem cells into motor neurons. Cell. 110, 385-397.
- Wonders, C., and Anderson, S.A. (2005). Cortical interneurons and their origins. Neuroscientist. 11, 199-205.
- Wu, H., Xu, J., Pang, Z.P., Ge, W., Kim, K.J., Blanchi, B., Chen, C., Sudhof, T.C., and Sun, Y.E. (2007). Integrative genomic and functional analyses reveal neuronal subtype differentiation bias in human embryonic stem cell lines. Proc Natl Acad Sci. 104, 13821-13826.
- Xie, W., Schultz, M.D., Lister, R., Hou, Z., Rajagopal, N., Ray, P., Whitaker, J.W., Tian, S., Hawkins, R.D., and Leung, D. (2013). Epigenomic analysis of multilineage differentiation of human embryonic stem cells. Cell. 153, 1134-1148.
- Xue, Y., Zhan, X., Sun, S., Karuppagounder, S.S., Xia, S., Dawson, V.L., Dawson, T.M., Laterra, J., Zhang, J., and Ying, M. (2018). Synthetic mRNAs drive highly efficient iPS cell differentiation to dopaminergic neurons. Stem Cells Transl Med. 8, 112-123.
- Yamamizu, K., Piao, Y., Sharov, A.A., Zsiros, V., Yu, H., Nakazawa, K., Schlessinger, D., and Ko, M.S.H. (2013). Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports. 1, 545-559.
- Yang, N., Chanda, S., Marro, S., Ng, Y.H., Janas, J.A., Haag, D., Ang, C.E., Tang, Y., Flores, Q., and Mall, M. (2017). Generation of pure GABAergic neurons by transcription factor programming. Nat Methods. 14, 621-628.
- Zhang, Y., Pak, C.H., Han, Y., Ahlenius, H., Zhang, Z., Chanda, S., Marro, S., Patzke, C., Acuna, C., and Covy, J. (2013). Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron. 78, 785-798.