Mol. Cells

Polycomb-Mediated Gene Silencing in Arabidopsis thaliana

Dong-Hwan Kim, and Sibum Sung

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Polycomb group (PcG) proteins are conserved chromatin regulators involved in the control of key developmental programs in eukaryotes. They collectively provide the transcriptional memory unique to each cell identity by maintaining transcriptional states of developmental genes. PcG proteins form multi-protein complexes, known as Polycomb repressive complex 1 (PRC1) and Polycomb repressive complex 2 (PRC2). PRC1 and PRC2 contribute to the stable gene silencing in part through catalyzing covalent histone modifications. Components of PRC1 and PRC2 are well conserved from plants to animals. PcG-mediated gene silencing has been extensively investigated in efforts to understand molecular mechanisms underlying developmental programs in eukaryotes. Here, we describe our current knowledge on PcG-mediated gene repression which dictates developmental programs by dynamic layers of regulatory activities, with an emphasis given to the model plant Arabidopsis thaliana.

Keywords: chromatin looping, epigenetics, gene silencing, polycomb, trithorax


In eukaryotes, DNA is packaged into a group of histone proteins to form nucleosome, the basic structural unit of chromatin. Dimers of four distinct histone proteins (H2A, H2B, H3, and H4) constitutes the histone octamer that is a structural scaffold for nucleosome. Because DNA is tightly packaged in nucleosome and nucleosome often exists as higher-order chromosome structure, transcriptional regulation in eukaryotes requires elaborate transcriptional control machinery.

In most eukaryotes, a single cell (zygote) is produced after fertilization and develops into functionally and morphologically distinct multicellular tissues and organs through successive cell divisions and differentiation. Multicellular differentiation is achieved even though each uniquely differentiated cell contains exactly the same genome context. This genetically identical but functionally specialized cell fate is an output of differential transcriptional control in each cell lineage. It has long been a fundamental question in biology how a single cell can differentiate into functionally specialized cells in multicellular eukaryotes.

An earlier important clue arose from genetic study using Drosophila as a model organism. Homeotic genes in Drosophila determine body identities during development. Mutations in homeotic genes commonly result in various ectopic developments of body patterns (i. e., legs grow in the place of antennae) (Simon et al., 1992; Soto et al., 1995; Struhl and Akam, 1985). Subsequent genetic analyses in Drosophila unveiled a group of genes required for a proper control of such homeotic genes, and they are collectively called Polycomb group (PcG) genes (Lewis, 1978). PcG proteins participate in cell fate determination through their role in maintaining silent states of homeotic genes during development. In addition, homologs of PcG proteins have also been identified from plants and vertebrates, and mutations in these genes commonly resulted in homeotic mutations (Akasaka et al., 1996; Goodrich et al., 1997). Therefore, PcG proteins appear to be evolutionarily well conserved transcriptional regulators that are pivotal in key developmental programs.

In this review, we discuss recent advances in understanding on the function of PcG proteins during various developmental programs, with a focus given to Arabidopsis as a plant model system.


Polycomb repressive complex 2 (PRC2)

PcG genes were originally isolated from genetic screens in Drosophila that were designed to identify genes involved in controlling homeotic gene expression (Simon and Kingston, 2013).

Studies using PcG proteins in Drosophila have served as a good model system to elucidate mechanistic details of PcG proteins in eukaryotes (Schwartz and Pirrotta, 2007; Simon and Kingston, 2009). Biochemical purification recognized four core components of PRC2 in Drosophila, Enhancer of zeste (E(z)), Extra sex combs (ESC), Suppressor of zeste 12 (Su(z)12), and Nucleosome-remodeling factor 55 kDa subunit (Nurf55) (Czermin et al., 2002; Muller et al., 2002). These four subunits mediate histone methyltransferase (HMTase) activity of PRC2 on H3K27. Each subunit has a distinct role: E(z) harbors a catalytic Su(var)3–9, Enhancer-of-zeste and Trithorax (SET) domain for histone methylation; ESC enhances the catalytic activity of E(z); Su(z)12 and Nurf55 are necessary for the nucleosome association (Cao et al., 2002) (Fig. 1). ESC is also able to bind to H3K27me3 histone mark, stabilizing and boosting the catalytic activity of PRC2. These coordinated activities of each PRC2 subunit reinforce self-propagation of H3K27me3 repressive marks at target chromatin during successive cell divisions and thus establishing stable chromatin contexts throughout mitosis (Margueron et al., 2009; Steffen and Ringrose, 2014).

Figure F1
Conserved components of PRC1 and PRC2 between Drosophila and Arabidopsis. Core components of Drosophila PRC2 and PRC1 (upper) and the homologous subunits found in Arabidopsis (bottom) were presented. Functional equivalents ...

All four core subunits of PRC2 exist in Arabidopsis. Homologs of Drosophila E(z), the H3K27 methyltransferase, include CURLY LEAF (CLF), SWINGER (SWN), and MEDEA (MEA); Three homologs of Su(z)12 are EMBRYONIC FLOWER 2 (EMF2), VERNALIZATION 2 (VRN2), FERTILIZATION INDEPENDENT SEED 2 (FIS2). MULTI-SUBUNIT SUPPRESSOR OF IRA 1–5 (MSI1-5) are homologs of NURF55. FERTILIZATION INDEPENDENT ENDOSPERM (FIE) is a sole homolog of ESC. Detailed information on the components of Arabidopsis PRC2 is shown in Fig. 1.

In Arabidopsis, PRC2 complexes are grouped into three distinctive complexes largely based on three Su(z)12 homologs (FIS2-PRC2, EMF2-PRC2, and VRN2-PRC2). MSI1 and FIE are constitutively expressed and thus serve as common subunits for all PRC2. On the other hand, expression of FIS2 and MEA are restricted in the female gametophyte and seed tissues, contributing to normal seed development (Kohler et al., 2003). For example, FIS2-PRC2 prohibits the endosperm formation in the absence of fertilization and represses endosperm proliferation after fertilization. EMF2-PRC2 notably contributes to the sporophytic development (i.e. floral organ). VRN2-PRC2 promotes floral transition in response to vernalization (long-term cold temperature) through the repression of a potent floral repressor, FLOWERING LOCUS C (FLC) in Arabidopsis (De Lucia et al., 2008; Kim et al., 2009). Many developmental genes are de-repressed in the mutants of PRC2 components, thus resulting in defects in various plant developmental programs, ranging from seed germination to floral transition (Kim et al., 2012; Muller and Goodrich, 2011). While FIS2-PRC2 controls proper development of endosperm (a part of seeds that does not develop further after germination), EMF2-PRC2 and VRN2-PRC2 are essential for plant development. There are clear functional redundancies between components of EMF2-PRC2 and VRN2-PRC2. Genetic analysis demonstrated that VRN2 and EMF2 are functionally redundant and so CLF and SWN are in Arabidopsis (Lafos et al., 2011; Tang et al., 2012). vrn2 emf2 or clf swn double mutants commonly exhibit undifferentiated embryo-like cell growth phenotypes (Chanvivattana et al., 2004; Schubert et al., 2005). Therefore, EMF2-PRC2 and VRN2-PRC2 are essential for ensuring proper cell differentiation and growth during plant development (Chanvivattana et al., 2004; Makarevich et al., 2006; Schubert et al., 2005; Tang et al., 2012). It is, however, still not clear how these three PRC2 complexes exert cooperative and/or distinctive controls over developmental genes in time- and tissue-specific manners in plants.

Major function of PRC2 is to repress transcription through depositing repressive histone marks (i.e. H3K27me3) on target chromatin (He et al., 2013; Pien and Grossniklaus, 2007). Accumulation of H3K27me3 mark at chromatin is highly correlated with the level of gene repression. High-throughput genomic approaches have significantly improved our understanding on the importance of PRC2-mediated H3K27me3 in plant development. About 4,400 genes are marked with H3K27me3, which account for 17% annotated genes in Arabidopsis. H3K27me3 marks are mostly localized to individual genes in Arabidopsis, unlike in animals where H3K27me3 is mainly enriched at heterochromatin region (Zhang et al., 2007). Interestingly, genome-wide profile of H3K27me3 mark is shown to be changed in time or tissue-dependent manners (Bouyer et al., 2011; He et al., 2012; Lafos et al., 2011; Roudier et al., 2011; Weinhofer et al., 2010). For example, H3K27me3-enriched loci are different in various tissues, such as shoot apical meristem (SAM), root, endosperm, and leaf tissues, indicating that H3K27me3 deposition is dynamically controlled during the development of different tissues.

Polycomb repressive complex 1 (PRC1)

The core subunits of PRC1 found in Drosophila are Posterior sex combs (Psc), drosophila RING (dRING), Polyhomeotic (Ph), and Polycomb (Pc) (Fig. 1). These are classified into two groups based on their biochemical function: One group (known as a writer group) has catalytic activity to mono-ubiquitinate histone H2A (Psc and dRING); the other group (known as a reader group) can recognize certain modified histone marks (Pc). Mono-ubiquitination of H2A is mediated by Psc and dRING proteins that belong to a subfamily of E3 ubiquitin ligases. Classically, E3 ubiquitin ligases play a role in the degradation of protein through 26S proteasome machinery (Smalle and Vierstra, 2004). Unlike classical E3 ubiquitin ligases, E3 ubiquitin ligases, Psc and dRING, in PRC1 do not trigger 26S proteasome-mediated protein degradation. Instead, mono-ubiquitination of histone H2A (H2Aub1) results in transcriptional repression and chromatin compaction (Wang et al., 2004; Weake and Workman, 2008). In mammals, the ubiquitination activity of RING1B, a homolog of dRING, is strongly enhanced by the association with BMI1, a Psc homolog, through the RING–RING formation (Ben-Saadon et al., 2006; Buchwald et al., 2006; Cao et al., 2005).

In Arabidopsis, three homologs of Psc (AtBMI1a, AtBMIb, and AtBMI1c) and two homologs of dRING (AtRING1a and AtRING1b) have been identified (Sanchez-Pulido et al., 2008; Xu and Shen, 2008). Several studies have validated the enzymatic activity of these E3 ubiquitin ligases catalyzing H2Aub1 modification (Bratzel et al., 2010; Li et al., 2011; Yang et al., 2013). Therefore, the H2Aub1 modification appears to be a common mechanism by PRC1 in eukaryotes.

For example, levels of H2Aub1 accumulation at PcG target genes, LEC1, FUS3, and STM, are significantly reduced in atring1a atring1b double mutants, despite that the levels of H3K27me3 accumulation remain unchanged (Bratzel et al., 2010; Xu and Shen, 2008). This indicates that PRC1 acts at the downstream of PRC2 and that H3K27me3 alone is to some extent not sufficient to trigger the silencing of common targets of PRC1 and PRC2. Similar result was also observed from the study of HOX gene regulation using embryonic stem cells (Cao et al., 2005; Eskeland et al., 2010). The reduction of H2Aub1 by knocking out of RING1B in mammalian cells caused the loss of HOX gene silencing while the level of H3K27me3 at HOX locus is not altered. Therefore, there is a clear epigenetic crosstalk between PRC1 and PRC2, and the coordinated function of two complexes are essential for the proper control of PcG target genes.

The second group (reader) in PRC1, Pc, physically links functional crosstalk between PRC2 and PRC1. Drosophila Pc protein contains N-terminal chromo-domain (a chromatin binding domain) and C-terminal C-BOX domain (involved in PRC1 complex assembly) (Bardos et al., 2000). Especially, chromo-domain of Pc protein binds to H3K27me3 histone mark, which is catalyzed by PRC2 (Bernstein et al., 2006b; Min et al., 2003). Therefore, the histone binding activity of Pc contributes to the physical link between PRC2 and PRC1.

Although E3 ubiquitin ligases (Psc and dRING) are well conserved across many eukaryotes, no apparent homolog of either Pc or Ph is found based on sequence homology in Arabidopsis genome. However, several functional equivalents of Drosophila Pc have been identified in Arabidopsis. One of them is a chromo-domain protein, LIKE-HETEROCHROMATIN PROTEIN (LHP1)/ TERMINAL FLOWER2 (TFL2) (Mylne et al., 2006; Sung et al., 2006). Like Pc in Drosophila, LHP1 harbors a chromo-domain at its N-terminal region. LHP1 displays the binding specificity to H3K27me3 histone mark through its chromo-domain (Exner et al., 2009). In addition, a genome-wide analysis of LHP1 showed that LHP1-enriched loci are highly overlapped with H3K27me3 enriched loci (Turck et al., 2007; Zhang et al., 2007). Another characteristic of LHP1 as a functional equivalent of Pc is that LHP1 physically interacts with AtRING1a, a component of PRC1 (Xu and Shen, 2008). Taken together, these data support the idea that LHP1 plays a role, similar to Drosophila Pc, as a reader component of PRC1 and forms a PRC1-like complex with AtRING1 and AtBMI1 proteins in Arabidopsis. Up to date, however, no functional homolog of Ph has been identified in Arabidopsis. Detailed information of components of PRC1 are shown in Fig. 1.

Two DNA-binding proteins, EMBRYONIC FLOWER1 (EMF1) and REDUCED VERNALIZATION RESPONSE 1 (VRN1) appear to function as components of PRC1 in Arabidopsis (Aubert et al., 2001; Calonje et al., 2008; Levy et al., 2002). Lesions in EMF1 resulted in pleiotropic phenotypes similar to mutants in PcG genes (Kim et al., 2012; Moon et al., 2003). EMF1 interacts with AtRING1a/1b and AtBMI1a/1b proteins in vitro and is required for proper H2Aub1 modification at PRC1 target chromatin (Bratzel et al., 2010). In vitro biochemical assay showed that EMF1 binds to DNA and inhibits the chromatin remodeling similar to the activity of Drosophila Psc (Beh et al., 2012; Calonje et al., 2008). A genomic-wide mapping using chromatinimmunoprecipitation (ChIP) followed by tiling-microarray analysis showed that EMF1 is highly enriched at loci marked with H3K27me3 (Kim et al., 2012). Therefore, it is likely that EMF1 is a component of PRC1 and cooperates with PRC2 through H3K27me3 histone mark. Because EMF1 does not show any sequence similarity with known functional domains of proteins, mechanistic details on how EMF1 exerts its function in Arabidopsis remain elusive. A B3-domain protein, VRN1 was identified from vernalization mutant screening in Arabidopsis (Levy et al., 2002). VRN1 is essential for the stable repression of the floral repressor, FLC, in response to vernalization, long-term cold. In vrn1 mutants, a repressive histone mark, H3K9me2 fails to accumulate at FLC, but H3K27me3 is normally enriched at FLC in vernalization, indicating that VRN1 is associated with H3K9 methylation at FLC (Bastow et al., 2004; Sung and Amasino, 2004). VRN1 binds to DNA in a non-sequence specific manner (Levy et al., 2002). It is unknown how VRN1 specifically participates in the repression of FLC in the absence of sequence specificity.

Several studies identified proteins that directly interact with LHP1. Up to date, EARLY IN SHORT DAYS7 (ESD7), INCURVATA2 (ICU2), SCARECROW (SCR) and SHORT VEGETATIVE PHASE (SVP), CYCLOPHILIN71 (AtCYP71), MULTICOPY SUPRESSOR OF IRA 1 (MSI1) and LHP1-interacting Factor 2 (LIF2) were shown to interact with LHP1 (Barrero et al., 2007; Cui and Benfey, 2009; del Olmo et al., 2010; Derkacheva et al., 2013; Latrasse et al., 2011; Li and Luan, 2011). Functional implication of these LHP1 interacting proteins remains obscure in regard to their roles in PRC1-mediated gene repression. However, diverse LHP1 interacting proteins indicates that LHP1 plays various roles in plant developmental programs through the interaction with a variety of regulatory proteins. Therefore, identification and characterization of PRC1 components would allow us to understand molecular details on PRC1 in plants.

TrxG complex antagonize PRC2

Trithorax group (TrxG) proteins activate gene expression through depositing active histone marks, H3K4me3 and H3K36me3 at target chromatin, which often overlap with PcG target chromatin, thus antagonize PcG function (Papp and Muller, 2006; Schwartz and Pirrotta, 2008). As in the case of PRC2, TrxG proteins are also well conserved in eukaryotes (Ringrose and Paro, 2004; Schuettengruber et al., 2007). In yeast, H3K4me3 is catalyzed by TrxG-containing complex called as complex proteins associated with Set1 (COMPASS) (Mohan et al., 2011). COMPASS-like complex is also conserved in other higher eukaryotes, such as mixed lineage leukemia (MLL)-complex in mammals.

H3K27me3 and its antagonistic H3K4me3 histone marks are generally localized around transcription start sites (TSSs) at target loci, likely competing with each other. In Drosophila, the presence of H3K4me3 and H3K36me3 inhibits the catalytic activity of PRC2 (Schmitges et al., 2011; Yuan et al., 2011).Similarly, histone acetylation at H3K27 (H3K27ac) directly inhibit methylation of H3K27 by PRC2, as both modifications cannot occur at the same residue (Pasini et al., 2010; Tie et al., 2009; 2014). Another layer of regulation of the antagonistic effect between PcG and TrxG proteins is mediated by H3K27 demethylases, such as Ultrathorax (UTX), which directly associate with COMPASS complex and enhance gene activation by the removal of H3K27me3 from target chromatin (Lee et al., 2007a; 2007b).

Similar antagonistic phenomena between PcG and TrxG proteins have been also reported in Arabidopsis. The first identified TrxG protein in Arabidopsis is ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1), a SET-domain protein. Mutations in ATX1 resulted in a number of pleiotropic phenotypes, including early flowering due to the severe reduction of H3K4me3 at FLC (Pien et al., 2008; Saleh et al., 2008). Subsequently, ATX1-like genes such as ARABIDOPSIS TRITHORAX-RELATED 3 (ATXR3)/SET DOMAIN GROUP 2 (SDG2) and ARABIDOPSIS TRITHORAX-RELATED 7 (ATXR7)/SET DOMAIN GROUP 25 (SDG25) are also found to act as H3K4me3 methyltransferases in Arabidopsis (Tamada et al., 2009; Yun et al., 2012). Another SET-domain gene, EARLY FLOWERING IN SHORT DAYS (EFS), a homolog of Drosophila absent small homeotic disks1 (ASH1) (Tripoulas et al., 1994), is also identified from mutant screening for early flowering in Arabidopsis (Soppe et al., 1999). H3K4me3 and H3K36me3 levels are decreased in efs mutants, indicating EFS acts as a transcriptional activator through catalyzing H3K4 and H3K36 methylation (Kim et al., 2005; Ko et al., 2010; Zhao et al., 2005). Antagonistic relationship between H3K36me3 and H3K27me3 is recently demonstrated in the cold-induced epigenetic control of FLC (Yang et al., 2014a), suggesting that antagonistic effect between TrxG and PcG proteins is also one of well-conserved phenomena in eukaryotes.

However, it should be noted that genome-wide analyses indicate the enrichment of H3K4me3 by TrxG and that of H3K27me3 by PRC2 are not always mutually exclusive. Rather, both histone marks do co-reside in a number of developmentally regulated genes in both animals and plants (Bernstein et al., 2006a; Jiang et al., 2008; Roudier et al., 2011; Zhang et al., 2007; 2009). These bivalent histone marks of H3K4me3 and H3K27me3 suggest that the regulation of numerous PcG/TrxG target genes can be achieved by delicate balances between H3K4me3 and H3K27me3 marks (Schwartz et al., 2010).

Like PcG complexes, TrxG proteins act in tissue- and time-dependent manners. A TrxG-interacting protein, WDR5, binds to a long non-coding RNAs (lncRNAs), HOXA TRANSCRIPT AT THE DISTAL TIP (HOTTIP) and NETTOIE SALMONELLA PAS THEILER’S (NeST), which in turn coordinate the activation of several TrxG target genes through H3K4 methylation (Gomez et al., 2013; Wang and Chang, 2011; Yang et al., 2014b). Up to date, no similar ncRNA has been identified in plants. Instead, a plant specific SAND domain protein, ULTRAPETALA1 (ULT1), has been reported to bind to ATX1 and may be responsible for guiding the ATX1-containing complex to target loci (Carles and Fletcher, 2009). It is still poorly understood how various TrxG complexes can be recruited onto their specific target loci.



Chromosomes, physical storage units of DNA, adopt highly organized structure and occupy distinct territories with preferential locations in the nucleus (Lanctot et al., 2007). Though several PRE DNA motif and lncRNAs are suggested as recruiting factors for PcG-containing complexes, our understanding on PREs- and lncRNAs-mediated PcG recruitment is still limited. Genome-wide studies in Drosophila showed that PcG proteins and discrete PRE DNA-elements cluster into large genomic domains, referred to as “Polycomb bodies (Pb)”, which are co-localized with H3K27me3 marks (Buchenau et al., 1998; Messmer et al., 1992; Pirrotta, 1997).

The development of chromosome conformation capture (3C) assay tool allows to measure physical and spatial interactions among discrete chromatin regions (Dekker et al., 2002). For example, PREs in the homeotic bithorax complex (BX-C) in Drosophila exist in contact with other PREs of repressed HOX genes by the formation of multiple chromatin loops (Lanzuolo et al., 2007). Immuno-fluorescent in situ hybridization (Immuno-FISH) assay showed that PcG-mediated gene silencing take places in Pb loci (Bantignies and Cavalli, 2011; Grimaud et al., 2006). Therefore, it is conceivable that PcG proteins and PREs act to create higher-order structures in nucleus, and these PcG-condensed Pb loci are critical to ensure proper gene silencing through the enrichment of H3K27me3 repressive marks at target chromatin (Cheutin and Cavalli, 2014; Schuettengruber et al., 2009; Schwartz et al., 2006; Sexton et al., 2012).

Higher-order chromatin structures are also observed at FLC, a common target of TrxG and PcG in Arabidopsis (Crevillen et al., 2013). Using 3C, a gene loop between the 5′ and 3′ flanking regions of the FLC locus appears to be correlated with the state of FLC transcription before vernalization. This loop is disrupted during vernalization, and the disruption of the loop coincides with the cold-induced FLC transcriptional shut-down. Mutations in BAF60, which encodes a SWI/SNF ATP-dependent chromatin remodeler, exhibit a stronger formation of this gene loop was detected and thus resulted in the up-regulation of FLC (Jegu et al., 2014).

The formation of higher-order of chromatin structure is apparent in the study of “FLC” body. Nuclear re-localization events at the FLC locus were observed when FLC is repressed by vernalization (Rosa et al., 2013). VRN5 encodes a PHD finger protein and is required for the PRC2-mediated silencing of FLC by vernalization. In vrn5 mutants, this nuclear re-localization by vernalization is abolished, indicating that the nuclear re-location is one of processes that occur during the vernalization-mediated stable silencing of FLC. Taken together, higher-order chromatin structural change is likely closely related to transcriptional states of PcG and TrxG target genes in Arabidopsis.


PRC2 and PRC1 play critical roles in the maintenance of stable transcriptional states of developmental genes through histone modifications, H3K27me3 and H2Aub1, respectively. PcG-mediated repressive marks are antagonized by TrxG complex, which catalyzes H3K4me3 and H3K36me3 histone marks. To ensure proper development, PcG- and TrxG-mediated regulation of gene expression need to be precisely programmed in time- and tissue-dependent manners. One of enigmas in the study of PcG- and TrxG-mediated regulation of gene expression is how these complexes are recruited to subsets of target genes according to developmental programs and environmental cues. Up to date, PRE cis-elements, transcription factors (TFs), and lncRNAs are proposed to contribute to the recruitment. It is expected that various mechanisms exist, and further identifications of PcG-interacting factors would extend our understanding and allow us to extract fundamental principles underlying epigenetic regulation of eukaryote developmental programs.

Article information

Mol. Cells.Dec 31, 2014; 37(12): 841-850.
Published online 2014-11-20. doi:  10.14348/molcells.2014.0249
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA
Received September 12, 2014; Accepted September 15, 2014.
Articles from Mol. Cells are provided here courtesy of Mol. Cells


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Figure 1

Conserved components of PRC1 and PRC2 between Drosophila and Arabidopsis. Core components of Drosophila PRC2 and PRC1 (upper) and the homologous subunits found in Arabidopsis (bottom) were presented. Functional equivalents of PcG complex are indicated with the same color. EMF1 and VRN1 are plant-specific proteins in Arabidopsis PRC1-like complex.

Figure 2

Several proposed modes of the recruitment of PcG and TrxG complexes. (A) PcG complexes, PRC1 or PRC2 physically interacts with transcription factors (TFs) or lncRNAs to be recruited to specific target loci, containing PRE. Recruited PRC1 and PRC2 catalyze H2Aub1 (red circle) and H3K27me3 (green triangle) on target loci, respectively. Some TFs (i.e. AG) competes PRC2 out of target loci in a time-dependent manner. (B) TrxG complex is recruited to target loci through the interaction with TFs or lncRNAs. Recruited TrxG complex catalyze H3K4me3 (blue triangle) or H3K36me3 (purple pentagon) which inhibit PRC2 activity. TrxG-recruiting lncRNAs or DNA elements have not been identified in Arabidopsis.