ADP-Ribosylation: Activation, Recognition, and Removal

Nan Li, and Junjie Chen

Abstract

ADP-ribosylation is a type of posttranslational modification catalyzed by members of the poly(ADP-ribose) (PAR) polymerase superfamily. ADP-ribosylation is initiated by PARPs, recognized by PAR binding proteins, and removed by PARG and other ADP-ribose hydrolases. These three groups of proteins work together to regulate the cellular and molecular response of PAR signaling, which is critical for a wide range of cellular and physiological functions.

Keywords: ADP-Ribosylation, PARG, PARP, PAR recognition domain

INTRODUCTION

ADP-ribosylation is a type of protein posttranslational modification initiated by a group of enzymes named poly(ADP-ribose) (PAR) polymerases (PARPs). The PARPs can hydrolyze nicotinamide adenine dinucleotide and transfer ADP-ribose to substrates. There are two types of ADP-ribosylation: mono-ADP-ribosylation is a modification in which only one unit of ADP-ribose is transferred to Glu/Asp/Lys residues of target proteins, while poly-ADP-ribosylation (PARsylation) involves several units of ADP-ribose being transferred to specific residues of target proteins to form ADP-ribose polymers (Hassa and Hottiger, 2008; Schreiber et al., 2006).

Posttranslational modification by PARsylation regulates cellular functions via several mechanisms. For example, ribosylation of some DNA repair proteins is required for their recruitment to DNA damage sites; ribosylation of substrate proteins can also affect protein-protein interactions, especially in the case of PAR-binding proteins that specifically recognize the ribosylated target proteins; ribosylation of target proteins can also facilitate the recruitment of E3 ubiquitin ligase, which promotes target protein degradation via proteasomal pathway. Through these distinct mechanisms, PARsylation participates in a wide range of cellular processes, including DNA damage response, transcription, chromatin remodeling, telomere protection, cell proliferation, cell death, and tumorigenesis. In this review, we summarize our current understanding of PARP family members and their functions in various aspects of cellular processes. In addition, we categorize the PAR recognition domains and describe the importance of PAR-binding motifs in signal transduction. Moreover, we introduce the unique functions of several enzymes that are specifically involved in the removal of ADP-ribosylation, which indicates the reversible nature of ADP-ribo-sylation as a signaling moiety.

THE PARP FAMILY MEMBERS AND THEIR DIVERSE CELLULAR FUNCTIONS

DNA damage-dependent PARPs

The DNA damage-dependent PARPs are normally activated by DNA breaks. PARP1, the first identified and the best understood PARP, is a nuclear protein and has three known functional domains: the DNA-binding domain at its very N-terminal region (Zinc finger domain); the auto-modification domain in the middle (i.e. BRCT domain), which is also where PARP1 interacts with its substrates as well as the region that undergoes auto-modification/poly-ribosylation; and the C-terminal catalytic domain, which binds to nicotinamide adenine dinucleotide and promotes the formation of ADP-ribose chain on its substrates as well as itself (Rouleau et al., 2010). PARP1 is required for DNA single-strand break repair (SSBR) and base-excision repair (BER) by binding to and recruiting XRCC1 to DNA damage site (Parsons et al., 2005). In addition, PARP1 also interacts with DNA double-strand break repair proteins such as Ku and DNA-PKcs and participates in NHEJ (nonhomologous end joining) repair pathway (Kraus, 2008; Li et al., 2004; Ruscetti et al., 1998). Thus, PARP1 inhibitors have been developed for cancer treatment, since they would sensitize tumors to radiation and/or chemotherapeutic agents that induce DNA strand breaks. More recently, PARP inhibition has been shown to cause synthetic lethality in homologous recombination deficient tumor cells, such as BRCA1 or BRCA2 deficient cells, by blocking base excision repair and therefore has been used in clinic trials for the treatment of BRCA-deficient tumors (Bryant et al., 2005; Farmer et al., 2005; Patel et al., 2011).

PARP2 can also be activated by DNA strand breaks (Ame et al., 1999). PARP1- and PARP2-knockout cells are sensitive to DNA damage, exhibiting marked increased chromatin instability and repair defects (Schreiber et al., 2002). PARP1 and PARP2 knockout mice display similar hypersensitivity to ionizing radiation (de Murcia et al., 2003), suggesting that both PARP1 and PARP2 are involved in DNA damage repair. Likewise, PARP3 is similar to PARP1 and PARP2, since its activity is also stimulated by DNA strand breaks. Recent study suggests that PARP3, and APLF (aprataxin and PNK-like factor) work cooperatively to facilitate double-strand break repair by promoting DNA ligation (Rulten et al., 2011). Therefore, this group of PARPs, PARP1/2/3, is activated by DNA damage and plays critical roles in DNA damage signaling and DNA repair.

PARP1 is the major PARP responding to DNA damage. However, comparing to PARP1 or PARP2 deficient mice, which are sensitive to DNA damage, the PARP1/2 double KO mice are embryonic lethal (de Murcia et al., 2003), indicating that PARP1 and PARP2 at least share some redundant functions in vivo. Whether PARP1/2/3 work together or separately in DNA damage repair remains unknown. In response to DNA damage, many DNA damage repair proteins as well as PARP1 are recruited to DNA damage sites. At the same time, PARP1 catalyzes ADP-ribosylation of its target proteins at sites of DNA damage. Although the connections between ADP-ribosylation and the recruitment for these DNA damage repair proteins have not been fully elucidated, recent studies suggest that a subset of BRCT and FHA domains may have PAR-binding activity and thus participate in damage-induced recruitment of DNA damage checkpoint and repair proteins (Li et al., 2013).

Tankyrases

Tankyrase1/PARP5a and tankyrase2/PARP5b are closely related, both having long ankyrin repeat regions at the N-terminal region, which are required for protein-protein interaction. They also contain sterile alpha modules in the middle, which is required for homodimerization and heterodimerization, and catalytic PARP domains at the C-terminus (Hsiao and Smith, 2008). The difference between these two proteins is that tankyrase 2 lacks the homopolymeric tracks of the His, Pro, and Ser domain with unknown function, which is present at the N-terminus of tankyrase 1. Otherwise, tankyrase 1 and tankyrase 2 have very similar domain structures and functions (Sbodio et al., 2002).

Tankyrases are known to have diverse functions by targeting different substrates for ADP-ribosylation. Researchers first identified that tankyrases affect telomere extension by PARsylating TRF1, which negatively regulates telomere extension (Smith et al., 1998). Mechanistically, it is known that tankyrases interact with and PARsylate TRF1, promoting the proteasomal degradation of TRF1 and thus leading to its release from telomeres to promote telomere extension (Smith and de Lange, 2000). Tankyrase 1 can also PARsylate nuclear mitotic apparatus protein (NUMA) to control sister chromatid cohesion (Chang et al., 2005). In addition, tankyrase 1 has also been shown to PARsylate Miki to promote prometaphase progression (Ozaki et al., 2012). Moreover, Tankyrase1 can PARsylate 3BP2, leading to its degradation, and thus facilitating normal bone formation (Levaot et al., 2011).

Tankyrases have recently been recognized as potential drug targets for cancer treatment (Riffell et al., 2012). Tankyrases PARsylate and promote the proteasome-dependent degradation of axin, which leads to the activation of the Wnt signaling pathway (Huang et al., 2009). Mechanistically, it is now known that the PAR-binding E3 ligase RNF146 mediates the ubiquitination and degradation of PARsylated axin (Zhang et al., 2011a). Moreover, Zhang and colleagues demonstrated that the tankyrase inhibitor XAV939 could stabilize axin1/2 and thus inhibit the Wnt signaling pathway (Huang et al., 2009). Therefore, tankyrase inhibitors are being actively pursued as possible anti-cancer agents (Riffell et al., 2012).

CCCH-type PARPs

The CCCH-type PARPs PARP7, PARP12, and PARP13 have a common Cys-Cys-Cys-His zinc finger domain and common WWE domains. PARP7/TiPARP is a mono-ADP-ribosyltransferase that is regulated by the aryl hydrocarbon receptor (AHR). This CCCH-type PARP co-localizes with the AHR in the nucleus and transcriptionally represses AHR (MacPherson et al., 2013). PARP12 is involved in the regulation of Venezuelan equine encephalitis virus (VEEV) replication (Atasheva et al., 2012). PARP13 is catalytically inactive; but an isoform of PARP13 lacking PARP domain (ZAP) has been shown to interact with viral RNA and prohibit viral RNA accumulation (Gao et al., 2002). Overall, this family of PAPRs has not been extensively studied. However, given the presence of CCCH Zinc finger domain and the PAR-binding WWE domain on this group of PARPs, it is likely that these PARPs may engage in some interesting PAR-dependent regulations that need to be further elucidated.

Macro-PARPs

Macro-PARPs are PARPs that contain Macro domains, which include PARP9, PARP14 and PARP15. The macro-PARP BAL1/PARP9 is a transcription modulator that is differentially expressed in diffuse large B-cell lymphoma cells (Juszczynski et al., 2006). More recently, BAL1/PARP9 and it partner E3 ligase BBAP have been shown to participate in DNA damage response by mediating protein ubiquitination, which is involved in the recruitment of double-strand break repair proteins 53BP1 and BRCA1 (Yan et al., 2013).

BAL2/PARP14 contains three macrodomains and a WWE domain. Similar to PARP9, PARP14 has also been suggested to regulate gene transcription. It interacts with STAT6 and increases STAT6 transcriptional activity (Mehrotra et al., 2011). BAL3/PARP15 contains two macrodomains. It can bind to mono-ADP-ribose or poly-ADP-ribose via its macrodomain and exhibit mono-ADP-ribosylation activity (Venkannagari et al., 2013). However, there is no known function of PARP15.

Other PARPs

There are a number of PARPs that do not share any common domain structures. These include PARP4, PARP6, PARP8, PARP10, PARP11, and PARP16. PARP4/vPARP is the largest protein in the PARP family and is a component of the vaults, which is 13 MDa ribonucleoprotein complex present in the cytoplasm of cells (Raval-Fernandes et al., 2005). PARP4/vPARP-deficient mice exhibited increased formation of carcinogen-induced tumors (Raval-Fernandes et al., 2005), indicating that PARP4 may have an unknown function in tumor suppression. PARP6 has mono-ADP-ribosylation activity and negatively regulates cellular proliferation (Tuncel et al., 2012). PARP8 and PARP11 also possess mono-ADP-ribosylation activity, but little is known about the functions of these two proteins (Scarpa et al., 2013). PARP10 lacks glutamate residue within its catalytic domain. It has mono-ADP-ribosylation activity but cannot promote poly-ADP-ribosylation (Kleine et al., 2008). This PARP interacts with Myc and inhibits c-Myc- and E1A-mediated transformation (Yu et al., 2005). PARP16 acts as a mono-ADP-ribosyltransferase and negatively regulates the protein level of karyopherin-β1 (Di Paola et al., 2012). PARP16 promotes ADP-ribosylation of PERK and IRE1α and is required for their activation during endoplasmic reticulum stress (Jwa and Chang, 2012). Overall, these PARPs have not been widely studied and we only have a glimpse of their cellular and physiological functions.

THE PAR RECOGNITION DOMAINS

The PAR-binding motif

The PAR-binding motif (PBM) is the first identified PAR recognition domain (Pleschke et al., 2000). It is present in most of the known PAR-binding proteins. The consensus PBM sequence is refined to an eight-amino-acid motif: [HKR]-X-X-[AIQVY]-[KR]-[AILV]-[FILPV] (Gagne et al., 2008). The PBM-containing proteins are involved in diverse cellular processes. For example, both P53 and P21 contain PBM and are key factors in the regulation of cell cycle progression and proliferation, especially in response to stresses like DNA damage (Fahrer et al., 2007). In addition, x-ray repair cross-complementing protein 1 (XRCC1) also has PBM and is rapidly recruited to DNA damage sites, where PARP1-mediated poly-ADP-ribosylation occurs (Pleschke et al., 2000). Apoptosis-inducing factor 1 (AIF1), another PBM-containing PAR-binding protein, is critically important for PARP1-induced cell death (Yu et al., 2006). Thus, many of these proteins are involved in certain aspects of DNA damage response.

The macrodomain

The macrodomain contains 140–190 residues and was initially discovered in histone variant macroH2A (Pehrson and Fried, 1992). The macrodomain-containing proteins currently have 10 members and can be categorized into four groups (Chen et al., 2011; Han et al., 2011) (Fig. 2). As discussed above, PARP9, PARP14, and PARP15, which make up the BAL1 family, contain two or three macrodomains. PARP14 and PARP15 exhibit mono-ADP-ribosylation activity while PARP9 seems to be inactive (Aguiar et al., 2005). The MacroD1, MacroD2, and C6orf130 have recently been shown to possess enzymatic activity and participate in the removal of mono-ADP-ribose from substrate proteins (Jankevicius et al., 2013; Rosenthal et al., 2013; Sharifi et al., 2013). Additionally, macroH2A1 and macroH2A2, which are histone subunits, function in transcriptional regulation (Changolkar et al., 2007; Costanzi and Pehrson, 2001). Finally, CHD1L is an ATP-dependent chromatin remodeler (Gottschalk et al., 2009), while GDAP2 is induced by ganglioside synthase expression (Neuvonen and Ahola, 2009). Besides MarcoD1/2 and C6orf130 that have terminal ADP-ribose protein glycohydrolase activity, the functional significance of other macrodomains has not been extensively studied.

Figure 2

Schematic diagrams of macro domain-containing proteins. The PARP is the catalytic domain and is required for NAD+ binding and PAR synthesis. Macro and WWE are PAR-binding domains. H2A is the ...

PAR-binding zinc finger

PAR-binding zinc finger (PBZ) is a recently identified zinc finger domain that binds to PAR (Ahel et al., 2008). There are three known PBZ-containing proteins. Checkpoint protein with FHA and RING domains (CHFR) is a tumor suppressor that functions in an early mitotic checkpoint by preventing cells from entering into mitosis (Scolnick and Halazonetis, 2000). APLF is also an FHA domain-containing protein that participates in DNA damage responsive pathway (Iles et al., 2007; Rulten et al., 2008). Mutation of PBZ domain in either CHFR or APLF abolishes their ability to bind to PAR (Ahel et al., 2008). Moreover, PBZ domain mutant of CHFR abrogates its function in mitotic checkpoint control (Ahel et al., 2008), but the underlying mechanism remains unclear. Another PBZ-containing protein in mammals is the DNA cross-link repair protein SNM1A (Oberoi et al., 2010).

The WWE domain

The WWE domain is the most interesting of the recently identified PAR-binding domains, as some WWE-containing proteins exhibit E3 ligase activity and are critical not only for the recognition of PARsylated proteins but also mediating ubiquitination and proteasomal degradation of these modified proteins (Aravind, 2001). The WWE domain-containing proteins can be categorized into three groups (Fig. 3). The first group consists of PARP7, PARP11, PARP12, PARP13, and PARP14, all of which have one or two WWE domains and a PARP domain (Wang et al., 2012). The second group consists of the E3-ubiquitin ligases Deltex1, Deltex2, Deltex4, HUWE1, TRIP12, and RNF146/Iduna. Deltex1, Deltex2, and Deltex4 are homologs of Drosophila Deltex, which is a critical regulator of Notch signaling (Matsuno et al., 1998). HUWE1 is the E3 ligase for apoptotic protein Mcl1 (Zhong et al., 2005). TRIP12 is thought to regulate the accumulation of RNF168 and downstream DNA damage pathways (Gudjonsson et al., 2012). Of the proteins in this group, only RNF146/Iduna has exhibited a functional connection between its E3 ligase activity and ADP-ribosylation. RNF146 protects neurons by interfering with PAR polymer-induced cell death (Andrabi et al., 2011). RNF146 also protects against cell death induced by DNA damage via PAR-dependent ubiquitination and proteasomal degradation of PARP1 (Kang et al., 2011). The most exciting finding regarding RNF146 is that it can regulate the WNT signaling pathway by mediating tankyrase-dependent degradation of axin. RNF146 binds directly to ADP-ribosylated axin and promotes axin ubiquitination and degradation (Zhang et al., 2011b). These findings raise the question whether or not other WWE domain-containing E3 ligases also target various PARP substrates for ubiquitination and degradation. Moreover, RNF146 only binds to poly-ADP-ribose, but not mono-ADP-ribose (Wang et al., 2012), indicating that there may be other yet-to-be-identified E3 ligases that recognize mono-ADP-ribose and mediate proteasome-dependent degradation of mono-ribosylated substrates.

Figure 3

Schematic diagrams of WWE domain-containing proteins. The PARP is the catalytic domain and is required for NAD+ binding and PAR synthesis. The sterile α-motif (SAM) is a protein-protein interaction domain. ...

The third group of WWE domain-containing proteins is actually just one protein: DDHD2. This protein has one WWE domain and one DDHD phospholipase domain and is involved in inter-cellular membrane trafficking at the Golgi complex/endoplasmic reticulum interface (Gonzalez et al., 2013).

BRCT and FHA domains

BRCT and FHA domains are known phosphopeptide binding domains that participate in DNA damage response and cell cycle regulations (Glover et al., 2004; Li et al., 2002; Yu et al., 2003). However, recent studies demonstrated that the FHA domains of APTX and PNKP, and the BRCT domains of BARD1, DNA Ligase 4, XRCC1 and NBS1 recognize Poly-ADP-ribose, and this PAR-binding ability is important for their recruitment to DNA damage sites (Li and Yu, 2013; Li et al., 2013). These studies further expand PAR-binding motifs and highlight the roles of ADP-ribosylation in signaling transduction.

PAR-REMOVING ENZYMES

Both mono-ADP-ribosylation and PARsylation can be reversed by PAR-removing enzymes. PAR glycohydrolase (PARG) is the best known PAR removing enzyme. It possesses both endoglycohydrolase and exoglycohydrolase activity and is responsible for hydrolysis of ribose-ribose bonds within poly-ADP-ribose chains (Ikejima and Gill 1988). PARG cleaves poly-ADP-ribose chains into free chains or mono-ADP-riboses (Lin et al., 1997; Slade et al., 2011). PARG has several isoforms owing to alternative splicing: a very active full-length 110-kDa nuclear protein, a shorter 102-kDa nuclear isoform, and a 60-kDa variant located in mitochondria due to the lack of an NLS (nuclear localization signal) at its N-terminal region. This mitochondrial variant of PARG cannot catalyze PAR degradation (Meyer-Ficca et al., 2004). PARG knockout leads to embryonic lethality in mice and PARG-deficient cells are sensitive to genotoxic stress (Koh et al., 2004), demonstrating critical functions of PARG in genome maintenance.

Another PAR-removing enzyme is the 39-kDa protein ADP-ribosyl hydrolase 3, which generates free ADP-ribose from the poly-ADP-ribose chain but cannot cleave the ADP-ribose-protein bond, therefore leading to the formation of mono-ADP-ribosylated proteins (Moss et al., 1992; Oka et al., 2006). The enzymes that can remove mono-ADP-ribose were unknown for a long time. Recently, several groups reported that some macrodomain-containing proteins, MacroD1, MacroD2, and C6orf130, have ADP-ribose hydrolase activity and can remove mono-ADP-ribose from substrate proteins by cleaving the ribose-protein bond (Jankevicius et al., 2013; Rosenthal et al., 2013; Sharifi et al., 2013), These findings significantly contributed to the understanding of PAR removal and recycle (Fig. 4).

Figure 4

Working hypothesis of sequential PAR removal by PARG and other ADP-ribose hydrolyases

CONCLUSION

ADP-ribosylation is one of the protein posttranslational modifications that has been studied for a long time. However, most of the studies focused on PARP1, which is the ubiquitous and best characterized member of the PARP family. Recent studies started to elucidate the biochemical activities and biological functions of other PARPs, which have greatly increased our knowledge of diverse functions of ADP-ribosylation in a variety of cellular processes.

The biology of ADP-ribosylation includes the synthesis, recognition, degradation, and recycling of this important biological moiety. All 17 PARPs are characterized by PARP domain, but they function differently in ADP-ribose synthesis (Scarpa et al., 2013). While PARP1-PARP4 and tankyrase1/2 have poly-ADP-ribosylation activity, PARP9 and PARP13 are thought to be catalytically inactive, while other PARPs exhibit mono-ADP-ribosylation activity. Besides the well-studied PARP1, researchers made exciting discoveries about the function of tankyrases in targeting axin degradation and activation of the Wnt pathway. However, for most PARPs, their functions, substrates, and how they are regulated are still largely unknown. Furthermore, the PAR recognition domains have now been appreciated as important mediators involved in signal transduction via ADP-ribosylation. Specifically, the PBM and a subset of FHA and BRCT domains are critically important for the recruitment of checkpoint and repair proteins to DNA damage sites, while the WWE domain of RNF146 is required for E3 ligase-mediated proteasomal degradation of ribosylated proteins. It remains to be determined whether there are new PAR-binding domains and PAR-binding proteins. On the other hand, PARG is the main enzyme that hydrolyzes ribose-ribose bonds. Recent discoveries that macrodomain-containing proteins have the activity to cleave mono-ADP-ribose from substrates provide another piece of the puzzle and underscore the reversible nature of ADP-ribosylation as a signaling moiety. How the activities of PARG and macrodomain-containing proteins are regulated and coordinated is another challenge the field is facing.

Article information

Mol. Cells.Jan 30, 2014; 37(1): 9-16.

Published online 2014-01-27. doi: 10.14348/molcells.2014.2245

Nan Li, and Junjie Chen^*

Department of Experimental Radiation Oncology, Unit 66, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA

^*Correspondence: jchen8@mdanderson.org

Received August 30, 2013; Accepted September 2, 2013.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.

Articles from Mol. Cells are provided here courtesy of Mol. Cells

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