Karyotype Analysis of a Korean Cucumber Cultivar (Cucumis sativus L. cv. Winter Long) Using C-banding and Bicolor Fluorescence in situ Hybridization

Mol. Cells 2002; 13(3): 413~418

Dal-Hoe Koo, Yoonkang Hur, Dong-Chun Jin, Jae-Wook Bang

ABSTRACT: An intensive karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) was car-ried out with three different methods. These included Feulgen staining, Giemsa C-banding, and fluorescence in situ hybridization (FISH). The mitotic chromosomes of the cucumber (2n = 2x = 14) were characterized, based on the length and arm ratio values. A C-banding analysis showed dark stains on the centromeric, te-lomeric, and intercalary regions of the chromosomes, except that chromosome 2 had a heavy staining in the long arm. Bicolor FISH, using 45S and 5S rDNA probes, provided additional information to identify cucumber chromosomes. The signals for 45S rDNA were detected on the pericentromeric regions of chro-mosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of chromosome 5. Similar band patterns (as the C-banding) were observed when the chromo-somes were counter-stained with 4?6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean cucumber cultivar is peculiar and different from previous reports.; Keywords: C-banding, Bicolor FISH, Karyo, Cucumis sativus L.

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30 September 2020 Volume 43,
Number 9, pp. 763~840

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