Mol. Cells 2001; 12(2): 149~157  
Replication of the Lagging Strand: A Concert of at Least 23 Polypeptides
Ulrich Huscher, Yeon-Soo Seo
© The Korean Society for Molecular and Cellular Biology. All rights reserved.

DNA replication is one of the most important events in living cells, and it is still a key problem how the DNA replication machinery works in its details. A replica-tion fork has to be a very dynamic apparatus since frequent DNA polymerase switches from the initiating DNA polymerase a to the processive elongating DNA polymerase d occur at the leading strand (about 8 ?104 fold on both strands in one replication round) as well as at the lagging strand (about 2 ?107 fold on both strands in one replication round) in mammalian cells. Lagging strand replication involves a very com-plex set of interacting proteins that are able to fre-quently initiate, elongate and process Okazaki frag-ments of 180 bp. Moreover, key proteins of this impor-tant process appear to be controlled by S-phase check-point proteins. It became furthermore clear in the last few years that DNA replication cannot be considered uncoupled from DNA repair, another very important event for any living organism. The reconstitution of nucleotide excision repair and base excision repair in vitro with purified components clearly showed that the DNA synthesis machinery of both of these macromo-lecular events are similar and do share many compo-nents of the lagging strand DNA synthesis machinery. In this minireview we summarize our current knowl-edge of the components involved in the execution and regulation of DNA replication at the lagging strand of the replication fork
Keywords: DNA Replicatio, Dna2, DNA Ligase 1, DNA Polymerase

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