Synaptotagmin 5 Controls SYP132-VAMP721/722 Interaction for Arabidopsis Immunity to Pseudomonas syringae pv tomato DC3000
Soohong Kim 1,4, Hyeran Kim 2,4, Keunchun Park 1,4, Da Jeong Cho 1, Mi Kyung Kim 1, Chian Kwon 1, and Hye Sup Yun 3,*
1Department of Molecular Biology, Dankook University, Cheonan 31116, Korea, 2Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Korea, 3Department of Biological Sciences, Konkuk University, Seoul 05029, Korea, 4These authors contributed equally to this work.
Received April 16, 2021; Revised July 11, 2021; Accepted August 8, 2021.; Published online September 10, 2021.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

ABSTRACT
Vesicle-associated membrane proteins 721 and 722 (VAMP721/722) are secretory vesicle-localized arginine-conserved soluble N-ethylmaleimide-sensitive factor attachment protein receptors (R-SNAREs) to drive exocytosis in plants. They are involved in diverse physiological processes in plants by interacting with distinct plasma membrane (PM) syntaxins. Here, we show that synaptotagmin 5 (SYT5) is involved in plant defense against Pseudomonas syringae pv tomato (Pst) DC3000 by regulating SYP132-VAMP721/722 interactions. Calcium-dependent stimulation of in vitro SYP132-VAMP722 interaction by SYT5 and reduced in vivo SYP132-VAMP721/722 interaction in syt5 plants suggest that SYT5 regulates the interaction between SYP132 and VAMP721/722. We interestingly found that disease resistance to Pst DC3000 bacterium but not to Erysiphe pisi fungus is compromised in syt5 plants. Since SYP132 plays an immune function to bacteria, elevated growth of surface-inoculated Pst DC3000 in VAMP721/722-deficient plants suggests that SYT5 contributes to plant immunity to Pst DC3000 by promoting the SYP132-VAMP721/722 immune secretory pathway.
Keywords: plant immunity, Pst DC3000, SYP132, SYT5, VAMP721/722
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31 August 2021 Volume 44,
Number 8, pp. 541~625
COVER PICTURE Heart-stage embryo dissected from Arabidopsis seed (Yoo et al., pp. 602-612).

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