Mol. Cells 2017; 40(12):   https://doi.org/10.14348/molcells.2017.0223
Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues
Sang Eun Ryu1,5, Tammy Shim1,5, Ju-Yeon Yi1, So Yeun Kim1,2, Sun Hwa Park3, Sung Won Kim3,
Gabriele V. Ronnett4, and Cheil Moon1,2,*
1Department of Cognitive and Brain Sciences, Graduate school, Daegu Gyeongbuk Institute of Science & Technology (DGIST),
Daegu 42988, Korea, 2Convergence Research Advanced Centre for Olfaction, Daegu Gyeongbuk Institute of Science & Technology
(DGIST), Daegu 42988, Korea, 3Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea,
College of Medicine, Seoul 06591, Korea, 4Departments of Neuroscience and Neurology, The Johns Hopkins University School
of Medicine, Baltimore, MD21205, USA, 5These authors contributed equally to this work.
*Correspondence: cmoon@dgist.ac.kr
Received September 19, 2017; Revised October 22, 2017; Accepted October 23, 2017.; Published online November 23, 2017.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

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ABSTRACT
Mammalian genomes are well established, and highly conserved regions within odorant receptors that are unique from other G-protein coupled receptors have been identified. Numerous functional studies have focused on specific conserved amino acids motifs, however, not all conserved motifs have been sufficiently characterized. Here, we identified a highly conserved 18 amino acid sequence motif within transmembrane domain seven (CAS-TM7) which was identified by aligning odorant receptor sequences. Next, we investigated the expression pattern and distribution of this conserved amino acid motif among a broad range of odorant receptors. To examine the localization of odorant receptor proteins, we used a sequence-specific peptide antibody against CAS-TM7 which is specific to odorant receptors across species. The specificity of this peptide antibody in recognizing odorant receptors has been confirmed in a heterologous in vitro system and a rat-based in vivo system. The CAS-TM7 odorant receptors localized with distinct patterns at each region of the olfactory epithelium; septum, endoturbinate and ectoturbinate. To our great interests, we found that the CAS-TM7 odorant receptors are primarily localized to the dorsal region of the olfactory bulb, coinciding with olfactory epitheliumbased patterns. Also, these odorant receptors were ectopically expressed in the various non-olfactory tissues in an evolutionary constrained manner between human and rats. This study has characterized the expression patterns of odorant receptors containing particular amino acid motif in transmembrane domain 7, and which led to an intriguing possibility that the conserved motif of odorant receptors can play critical roles in other physiological functions as well as olfaction.
Keywords: conserved, GPCR, odorant receptor, olfaction,
transmembrane


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30 November 2017 Volume 40,
Number 11, pp. 805~887

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