Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability
Sanghun Yeou 1 and Nam Ki Lee 2,*
1Department of Physics, Pohang University of Science and Technology, Pohang 37673, Korea, 2Department of Chemistry, Seoul National University, Seoul 08826, Korea
Received July 9, 2021; Revised July 19, 2021; Accepted July 20, 2021.; Published online September 2, 2021.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

ABSTRACT
The various DNA-protein interactions associated with the expression of genetic information involve double-stranded DNA (dsDNA) bending. Due to the importance of the formation of the dsDNA bending structure, dsDNA bending properties have long been investigated in the biophysics field. Conventionally, DNA bendability is characterized by innate averaging data from bulk experiments. The advent of single-molecule methods, such as atomic force microscopy, optical and magnetic tweezers, tethered particle motion, and single-molecule fluorescence resonance energy transfer measurement, has provided valuable tools to investigate not only the static structures but also the dynamic properties of bent dsDNA. Here, we reviewed the single-molecule methods that have been used for investigating dsDNA bendability and new findings related to dsDNA bending. Single-molecule approaches are promising tools for revealing the unknown properties of dsDNA related to its bending, particularly in cells.
Keywords: atomic force microscopy, DNA bending, DNA cyclization assay, D-shaped DNA, fluorescence resonance energy transfer, magnetic tweezers, optical tweezers, single-molecule, tethered particle motion


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31 August 2021 Volume 44,
Number 8, pp. 541~625
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