Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology
Joji Marie Teves 1,2 and Kyoung Jae Won 1,2,*
1Biotech Research and Innovation Centre (BRIC), University of Copenhagen, DK-2200 Copenhagen, Denmark, 2Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), Faculty of Health Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark
Received January 14, 2020; Revised April 22, 2020; Accepted May 10, 2020.; Published online June 4, 2020.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

ABSTRACT
Complex cell-to-cell communication underlies the basic processes essential to homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.
Keywords: cellular communication, single-cell RNA, spatial transcriptomics, tissue architecture


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30 June 2020 Volume 43,
Number 6, pp. 501~590

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