Chromatin Interacting Factor OsVIL2 Is Required for Outgrowth of Axillary Buds in Rice
Jinmi Yoon1, Lae-Hyeon Cho1,2, Sichul Lee3, Richa Pasriga1, Win Tun1, Jungil Yang1, Hyeryung Yoon1, Hee Joong Jeong1, Jong-Seong Jeon1, and Gynheung An1,*
1Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea, 2Department of Plant Bioscience, Pusan National University, Miryang 50463, Korea, 3Center for Plant Aging Research, Institute for Basic Science, Daegu 42988, Korea
Received June 25, 2019; Revised October 17, 2019; Accepted October 29, 2019.; Published online November 27, 2019.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

Shoot branching is an essential agronomic trait that impacts on plant architecture and yield. Shoot branching is determined by two independent steps: axillary meristem formation and axillary bud outgrowth. Although several genes and regulatory mechanism have been studied with respect to shoot branching, the roles of chromatin-remodeling factors in the developmental process have not been reported in rice. We previously identified a chromatin-remodeling factor OsVIL2 that controls the trimethylation of histone H3 lysine 27 (H3K27me3) at target genes. In this study, we report that loss-of-function mutants in OsVIL2 showed a phenotype of reduced tiller number in rice. The reduction was due to a defect in axillary bud (tiller) outgrowth rather than axillary meristem initiation. Analysis of the expression patterns of the tiller-related genes revealed that expression of OsTB1, which is a negative regulator of bud outgrowth, was increased in osvil2 mutants. Chromatin immunoprecipitation assays showed that OsVIL2 binds to the promoter region of OsTB1 chromatin in wild-type rice, but the binding was not observed in osvil2 mutants. Tiller number of double mutant osvil2 ostb1 was similar to that of ostb1 , suggesting that osvil2 is epistatic to ostb1. These observations indicate that OsVIL2 suppresses OsTB1 expression by chromatin modification, thereby inducing bud outgrowth.
Keywords: bud outgrowth, chromatin modification, rice
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30 November 2019 Volume 42,
Number 11, pp. 739~819

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