IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells
Bo Feng1,2, Qian Zhang1,2, Jianfang Wang1, Hong Dong1, Xiang Mu1, Ge Hu1,*, and Tao Zhang1,*
1Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University
of Agriculture, Beijing 102206, P.R. China, 2Department of Pathophysiology, College of Veterinary Medicine, China Agricultural
University, Beijing 100193, P.R. China
*Correspondence: bnhuge@126.com (GH); zhangtao8008@126.com (TZ)
Received June 1, 2017; Revised December 9, 2017; Accepted January 7, 2018.; Published online April 5, 2018.
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IFIT1 (also known as ISG56) is a member of the interferoninducible protein with tetratricopeptide repeats (IFITs) family. IFITs are strongly induced by type I interferon (IFN), doublestranded RNA and virus infection. Here, we investigated IFIT1 expression in human umbilical vein endothelial cells (HUVECs) and in human bronchus epithelial cells (BEAS-2Bs) induced by the H9N2 virus and inactivated viral particle at different time points. We also investigated the effect of H9N2 virus and viral particle infection on IFN-α/β production, and assessed whether hemagglutinin or neuraminidase protein induced IFIT1 expression. Results showed that both H9N2 virus infection and viral particle inoculation induced the expression of IFIT1 at mRNA and protein levels in the two cell lines. Hemagglutinin or neuraminidase protein binding alone is not sufficient to induce IFIT1 expression. Surprisingly, the expression patterns of IFIT1 in response to H9N2 virus and viral particles in the two cell lines were opposite, and production kinetics of IFN- α/β also differed. An additional finding was that induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Our data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection.
Keywords: endothelial cells, H9N2 virus, IFIT1 expression pattern, IFN-α/β

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