Mol. Cells 2019; 42(2): 135~142
OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells
Sang-Hun Choi1,2, Jun-Kyum Kim1,2, Hee-Young Jeon1,2, Kiyoung Eun1,2, and Hyunggee Kim1,2,*
1Department of Biotechnology, College of Life Sciences and Biotechnology, 2Institute of Animal Molecular Biotechnology, Korea University, Seoul 02841, Korea
Received July 23, 2018; Revised December 10, 2018; Accepted December 12, 2018.; Published online January 2, 2019.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

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OCT4, also known as POU5F1 (POU domain class 5 transcription factor 1), is a transcription factor that acts as a master
regulator of pluripotency in embryonic stem cells and is one of the reprogramming factors required for generating induced
pluripotent stem cells. The human OCT4 encodes three isoforms, OCT4A, OCT4B, and OCT4B1, which are generated
by alternative splicing. Currently, the functions and expression patterns of OCT4B remain largely unknown in malignancies,
especially in human glioblastomas. Here, we demonstrated the function of OCT4B in human glioblastomas. Among the
isoform of OCT4B, OCT4B-190 (OCT4B19kDa) was highly expressed in human glioblastoma stem cells and glioblastoma
cells and was mainly detected in the cytoplasm rather than the nucleus. Overexpression of OCT4B19kDa promoted colony
formation of glioblastoma cells when grown in soft agar culture conditions. Clinical data analysis revealed that patients
with gliomas that expressed OCT4B at high levels had a poorer prognosis than patients with gliomas that expressed
OCT4B at low levels. Thus, OCT4B19kDa may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.
anchorage-independent growth, cytoplasmic localization, glioblastoma, mechanical stress response, OCT4B

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31 January 2019 Volume 42,
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