DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells
Thao Dang1, Soo Hwa Jang1, Sung Hoon Back1, Jeong Woo Park1,*, and In-Seob Han1,*
1School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea
*Correspondence: hanis@ulsan.ac.kr (ISH); jwpark@ulsan.ac.kr (JWP)
Received March 15, 2018; Revised September 17, 2018; Accepted September 27, 2018.; Published online November 14, 2018.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (http://creativecommons.org/licenses/by-nc-sa/3.0/).
ABSTRACT
The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased ‘slow and long-lived’ subpopulations, but decreased ‘fast and short-lived’ subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells.
Keywords: DRG2, microtubule dynamics, tau


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30 November 2018 Volume 41,
Number 11, pp. 933~992

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