Tectorigenin Promotes Osteoblast Differentiation and in vivo Bone Healing, but Suppresses Osteoclast Differentiation and in vivo Bone Resorption
So-Youn Lee1,5, Gyu-Tae Kim2,5, Hyung-Mun Yun1, Youn-Chul Kim3, Il- Keun Kwon4, and
Eun-Cheol Kim1,*
1Department of Oral and Maxillofacial Pathology, 2Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyung
Hee University, Seoul 02453, Korea, 3Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang
University, Iksan00000, Korea, 4Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 02453, Korea,
5These authors contributed equally to this work.
*Correspondence: eckim@khu.ac.kr
Received January 31, 2018; Accepted February 2, 2018.; Published online May 15, 2018.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

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ABSTRACT
Although Tectorigenin (TG), a major compound in the rhizome of Belamcanda chinensis, is conventionally used for the treatment of inflammatory diseases, its effects on osteogenesis and osteoclastogenesis have not been reported. The objective of this study was to investigate the effects and possible underlying mechanism of TG on in vitro osteoblastic differentiation and in vivo bone formation, as well as in vitro osteoclast differentiation and in vivo bone resorption. TG promoted the osteogenic differentiation of primary osteoblasts and periodontal ligament cells. Moreover, TG upregulated the expression of the BMP2, BMP4, and Smad-4 genes, and enhanced the expression of Runx2 and Osterix. In vivo studies involving mouse calvarial bone defects with μCT and histologic analysis revealed that TG significantly increased new bone formation. Furthermore, TG treatment inhibited osteoclast differentiation and the mRNA levels of osteoclast markers. In vivo studies of mice demonstrated that TG caused the marked attenuation of bone resorption. These results collectively demonstrated that TG stimulated osteogenic differentiation in vitro, increased in vivo bone regeneration, inhibited osteoclast differentiation in vitro, and suppressed inflammatory bone loss in vivo. These novel findings suggest that TG may be useful for bone regeneration and treatment of bone diseases.
Keywords: bone remodeling, differentiation, osteoblast, osteoclast, tectorigenin


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