Mol. Cells 2017; 40(4): 271~279  https://doi.org/10.14348/molcells.2017.2308
Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development
Kyeong-Won Yoo1, Maivannan Thiruvarangan1, Yun-Mi Jeong1, Mi-Sun Lee1, Sateesh Maddirevula1, Myungchull Rhee1, Young-Ki Bae2, Hyung-Goo Kim3, and Cheol-Hee Kim1,*
1Department of Biology, Chungnam National University, Daejeon 34134, Korea, 2Comparative Biomedicine Research Branch, Research Institute, National Cancer Center, Goyang 10408, Korea, 3Department of OB/GYN, Department of Neuroscience and Regenerative Medicine, Augusta University, GA 30912, USA
*Correspondence: zebrakim@cnu.ac.kr
Received December 12, 2016; Revised March 20, 2017; Accepted March 21, 2017.; Published online March 28, 2017.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (http://creativecommons.org/licenses/by-nc-sa/3.0/).
ABSTRACT
Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bombbinding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.
Keywords: mind bomb, notch, RanBP9, retinogenesis, zebrafish


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