Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast
Young-Yon Kwon1, Sung-Keun Lee2, and Cheol-Koo Lee1,*
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea, 2Department of Pharmacology, College of Medicine, Inha University, Incheon 22212, Korea
*Correspondence: cklee2005@korea.ac.kr
Received November 16, 2016; Revised March 26, 2017; Accepted April 7, 2017.; Published online April 20, 2017.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (http://creativecommons.org/licenses/by-nc-sa/3.0/).
ABSTRACT
Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. Many effects of CR may contribute to extend lifespan. Specifically, CR prevents oxidative damage from reactive oxygen species (ROS) by enhancing mitochondrial function. In this study, we characterized 33 single electron transport chain (ETC) gene-deletion strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. Interestingly, defects in 17 of these 33 ETC genedeleted strains showed loss of both respiratory function and CR-induced CLS extension. On the contrary, the other 16 respiration-capable mutants showed increased CLS upon CR along with increased mitochondrial membrane potential (MMP) and intracellular adenosine triphosphate (ATP) levels, with decreased mitochondrial superoxide generation. We measured the same parameters in the 17 non-respiratory mutants upon CR. CR simultaneously increased MMP and mitochondrial superoxide generation without altering intracellular ATP levels. In conclusion, respiration is essential for CLS extension by CR and is important for balancing MMP, ROS, and ATP levels.
Keywords: caloric restriction, chronological lifespan, electron transport chain, mitochondria, respiration


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31 March 2017 Volume 40,
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