Inhibition of the DevSR Two-Component System by Overexpression of Mycobacterium tuberculosis PknB in Mycobacterium smegmatis
Hyun-Jung Bae1,4, Ha-Na Lee1,4, Mi-Na Baek1, Eun-Jin Park1, Chi-Yong Eom2, In-Jeong Ko3,
Ho-Young Kang1, and Jeong-Il Oh1,*
1Department of Microbiology, Pusan National University, Busan 46241, Korea, 2Korea Basic Science Institute, Seoul 02855, Korea,
3Korea Science Academy of KAIST, Busan 47162, Korea, 4These authors contributed equally to this work.
*Correspondence: joh@pusan.ac.kr
Received May 11, 2017; Revised July 11, 2017; Accepted August 25, 2017.; Published online September 30, 2017.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

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ABSTRACT
The DevSR (DosSR) two-component system, which is a major regulatory system involved in oxygen sensing in mycobacteria, plays an important role in hypoxic induction of many genes in mycobacteria. We demonstrated that overexpression of the kinase domain of Mycobacterium tuberculosis (Mtb) PknB inhibited transcriptional activity of the DevR response regulator in Mycobacterium smegmatis and that this inhibitory effect was exerted through phosphorylation of DevR on Thr180 within its DNA-binding domain. Moreover, the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.
Keywords: DevSR, mycobacterium, Ser/Thr protein kinase,
two-component system


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