Molecules and Cells

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Fig. 3.

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Fig. 3. TGFβ1-activated Smad3 binds to the MUC5AC promoter. ChIP assay using the anti-Smad3 antibody on the MUC5AC promoter region. The binding of Smad3 to the MUC5AC promoter was increased by TGFβ1 addition in NCI-H292 cells. Upper diagram: The MUC5AC promoter between -1384 and +31 bp contains two responsive elements (RE) for transcription factors such as Smad3 and NF-κB, predicted by the TFBIND. Lower panels: NCI-H292 cells were treated with each indicated concentration of TGFβ1 for 30 min. PCR primers were designed to identify the Smad3 RE within the MUC5AC promoter region between –234 to –134 bp (arrows in the upper diagram). Rabbit IgG was used as a negative control. Input samples (unprecipitated chromatin) were used as positive controls for PCR amplification.
Mol. Cells 2021;44:38~49
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