Molecules and Cells

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Fig. 2.

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Fig. 2. TGFβ1 negatively affects MUC5AC expression at the transcriptional level. (A) No adverse effect of TGFβ1 on NCI-H292 cell viability at concentration ranges of 0 to 40 ng/ml. (B and C) The effects of TGFβ1 on MUC5AC secretion were analyzed by ELISA. In NCI-H292 airway cells, TGFβ1 decreased MUC5AC secretion in a concentration- (B) and time- (C) dependent manner. NCI-H292 cells were incubated with an increasing or a fixed concentration of TGFβ1 for 24 h. (D) The effects of TGFβ1 on MUC5AC mRNA expression were evaluated using qRT-PCR. NCI-H292 cells were pretreated with various concentrations of TGFβ1 for 12 h. The expression of MUC5AC transcripts was more prominently reduced by TGFβ1 addition, compared with the secretion of MUC5AC proteins in Fig. 2B. Data represent mean ± SD of at least three individual experiments (*P < 0.05, **P < 0.01, and ***P < 0.001 compared with the control, without TGFβ1).
Mol. Cells 2021;44:38~49
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