Molecules and Cells

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Fig. 6.

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Fig. 6. Knockdown of ana3 decreases the level of Sona in both intra- and extracellular regions of wing discs. (A-F) Loss of ana3 decreases the level of Sona in both intracellular and extracellular regions. Sona is visualized in the wing discs of CS and ana3SH0558 3rd instar larvae with Sona-Pro antibody. Dlg is visualized with Dlg antibody (Cho et al., 2000) to show no change in the protein level unlike Ana3. Intracellular Sona in the apical disc proper (A and B) and extracellular Sona in the peripodial epithelium (C and D) and the basal region (E and F) of CS and ana3SH0558 wing discs were compared. (G and H) CS and ana3i flies were crossed with ci-Gal4 flies and dissected at late 3rd instar larval stage for extracellular immune-staining of Sona with Sona-Pro antibody. For more precise comparison, regions inside the white boxes in (G and H) were magnified (G’ and H’). Sona visualized by Sona-C antibody is presented in Supplementary Fig. S6, and the intensities of these wing discs were measured by ImageJ and plotted in (I). Scale bars = 50 μm (A-F); 33 μm (G and H); 11 μm (G’ and H’). **P < 0.01, ***P < 0.001.
Mol. Cells 2021;44:13~25
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