Molecules and Cells

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Fig. 5.

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Fig. 5. Ana3 promotes cell survival. (A) Number of adult survivors correlates with the amount of Ana3. Ubq-ana3-GFP, CS, and ana3SH0558/CyO-GFP larvae were irradiated with 1,000, 1,250, and 1,500 rad during 2nd to 3rd instar larval stages, and the number of adults was divided by the number of total empty pupal cases to obtain survival rates. The actual survival rates of unirradiated Ubq-ana3-GFP, CS, and ana3SH0558/CyO-GFP are indicated with the darker set of bars. To compare the survival rates of these flies after irradiation, the survival rate of the irradiated flies was divided by that of the unirradiated flies to obtain a normalized survival rate. Three independent experiments were carried out with more than 100 flies for each genotype and irradiation intensity. (B and C) Ana3 reduces the amount of activated Dcp1. CS and Ubq-ana3-GFP 3rd instar larvae were dissected 24 h after irradiation. (D and E) Irradiation increases the level of Ana3 in the wing discs of irradiated flies. CS 3rd instar larvae were dissected before and right after irradiation, as well as 1 h, 3 h, and 6 h after irradiation, and their wing discs were immuno-stained to visualize Ana3 (D). The fluorescence intensity of each imaginal disc was measured with ImageJ and averaged (n ≥ 15 for each) (E). Ctrl, control. Scale bar = 100 μm (B-D). *P < 0.1, **P < 0.01, ***P < 0.001.
Mol. Cells 2021;44:13~25
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