Molecules and Cells

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Fig. 4.

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Fig. 4. Loss of ana3 induces apoptosis. (A-D) Knockdown of ana3 induces cell death but the coexpression of p35 rescues the cell death phenotype. CS, UAS-ana3i, UAS-p35, and UAS-ana3i; UAS-p35 lines were crossed with the en-Gal4 UAS-GFP driver and incubated at 29°C. Apoptotic cells in the wing discs of larval progenies are visualized with Dcp-1. The Gal4 expressing region is marked with GFP. (E-I) The small wing phenotype by knockdown of ana3 (F) is rescued by coexpression of p35 (H). Adult wings of the controls are shown in (E) and (G). Flies were crossed and incubated at 29°C. Wing sizes were measured and averaged in the same way as in Fig. 2J (n > 10 for each) (I). The en>GFP, p35 wings were small and defective, similar to previous reports that overexpression of p35 causes developmental defects in eyes and wings (Katanayeva et al., 2010; Nguyen et al., 2016). Scale bars = 50 μm (A-D); 239 μm (E-H). *P < 0.1, **P < 0.01, ***P < 0.001.
Mol. Cells 2021;44:13~25
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