Molecules and Cells

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Fig. 1.

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Fig. 1. Crosstalk between ECs and lung cancer cells causes chemoresistance in lung cancer spheroids. (A) Experimental schematic of CM obtained from cultured HUVECs (ECs), lung cancer cells (NCI-H460 or A549), and lung cancer cells co-cultured with HUVECs. The cells were cultured for 3 days in 2D culture conditions using the same number of cells and the same amount of media. Next, three different CM were added to lung cancer cells in 3D culture conditions for 2 days. The spheroids were then treated with drugs for 2 days, and EthD-1 staining was used to identify dead cells. (B and C) Immunofluorescence images of lung cancer spheroids grown in CM obtained from cultured HUVECs, cultured lung cancer cells, or lung cancer cells co-cultured with HUVECs. The cells were treated with 5, 10, 20, or 40 μM gefitinib (B) or cisplatin (C) for 2 days, and then stained with 4 μM EthD-1. The images were obtained using the Operetta® High Content Screening System, and the intensity of EthD-1 staining in lung cancer spheroids relative to controls was analyzed using Harmony software. The data shown are the mean ± SD from three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the control group.
Mol. Cells 2021;44:50~62 https://doi.org/10.14348/molcells.2020.0212
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