Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 4.

Download original image
Fig. 4. Aryl sulfonamide negatively regulates transcriptional activity of ARNT. (A) HepG2 cells were transfected with pRL-TK, a XRE luciferase reporter. After 24 h, cells were treated with DMSO or indisulam (10 μM) along with TCDD (10 nM) for 12 h. Luciferase activity was measured with the Dual-Luciferase Reporter Assay System. Data are presented as mean ± SD (n = 3). *P < 0.001. (B) HepG2 cells were treated with DMSO, indisulam (1 μM) or E7820 (1 μM) in the presence or absence of TCDD (10 nM) for 12 h. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (C) HEK293T cells were transfected with pRL-TK, a HRE luciferase reporter. After 24 h, cells were treated with indisulam (10 μM) under normoxia or hypoxia for 12 h and luciferase activities measured with a Dual-Luciferase Reporter Assay System. Data are presented as mean ± SD (n = 3). *P < 0.001, ***P < 0.05. (D) HEK293T cells were treated with DMSO, indisulam (1 μM) or E7820 (1 μM) under normoxia or hypoxia for 12 h. Cell lysates were analyzed via immunoblotting with the indicated antibodies. (E-H) Total mRNAs from HEK293T cells treated with DMSO or indisulam (1 μM) under normoxia or hypoxia for 12 h were subjected to RT-qPCR analysis for the indicated HIF target genes. GLUT1, glucose transporter 1; LDHA, lactate dehydrogenase A; VEGF, vascular endothelial growth factor; TGFα, transforming growth factor α; Data are presented as mean ± SD (n = 3). *P < 0.001, **P < 0.01, ***P < 0.05.
Mol. Cells 2020;43:935~944 https://doi.org/10.14348/molcells.2020.0122
© Mol. Cells