Molecules and Cells

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Fig. 3.

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Fig. 3. Aryl sulfonamides induce ARNT degradation through CRL4DCAF15. (A) HEK293T cells were treated with the indicated concentrations of indisulam or E7820 for 24 h. Cell lysates were analyzed via immunoblotting with indicated antibodies. (B) HEK293T cells were treated with indisulam (1 μM) for the indicated time-periods. Cell lysates were analyzed via immunoblotting with the indicated antibodies. (C) HEK293T cells were treated with indisulam (1 μM) along with bortezomib (1 μM) or MLN4924 (1 μM) for 12 h. Cell lysates were analyzed via immunoblotting with the indicated antibodies. (D) HEK293T cells were transfected with FLAG-ubiquitin. After 24 h, transfected cells were co-treated with DMSO or indisulam and bortezomib for 12 h. Whole-cell lysates were immunoprecipitated using FLAG antibody-conjugated magnetic beads and immune complexes analyzed by immunoblotting with anti-ARNT antibody. (E) shControl or shDCAF15 HEK293T cells were treated with the indicated concentrations of indisulam for 24 h. Cell lysates were analyzed via immunoblotting with the indicated antibodies. (F) HEK293T cells were transfected with siControl or siRNA against RBM39 and RBM23. After 48 h, transfected cells were treated with the indicated concentrations of indisulam for 24 h. Cell lysates were analyzed via immunoblotting with the indicated antibodies. (G) HEK293T cells were transfected with DCAF15-HA, followed by treatment with DMSO or indisulam (1 μM) and bortezomib (1 μM) for 12 h. Cell lysates were immunoprecipitated using HA antibody-conjugated magnetic beads and immune complexes analyzed via immunoblotting with the indicated antibodies.
Mol. Cells 2020;43:935~944 https://doi.org/10.14348/molcells.2020.0122
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