Molecules and Cells

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Fig. 1.

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Fig. 1. ARNT interacts with DCAF15. (A) HEK293T cells were co-transfected with DCAF15-HA and MYC-ARNT. After 48 h, cell lysates were immunoprecipitated using MYC or HA antibody-conjugated magnetic beads. Immune complexes were analyzed by immunoblotting with the indicated antibodies. (B) HEK293T cells were transfected with DCAF15-HA. After 48 h, cell lysates were immunoprecipitated using HA antibody-conjugated magnetic beads. Immune complexes were analyzed by immunoblotting with the indicated antibodies. (C) HEK293T cells were transfected with MYC-ARNT and HA-full-length or truncated DCAF15 (1-300 aa: NTD or 301-600 aa: CTD). After 48 h, cell lysates were immunoprecipitated using HA antibody-conjugated magnetic beads. Immune complexes were analyzed by immunoblotting with the indicated antibodies (upper panel). Schematic diagram illustrating DCAF15 deletion mutants (lower panel). Asterisk (*) indicates a non-specific band. (D) HEK293T cells were co-transfected with DCAF15-HA and MYC-full length or truncated ARNT (1-467 aa, 1-264 aa, 1-171 aa, or 1-111 aa). After 48 h, cell lysates were immunoprecipitated using MYC antibody-conjugated magnetic beads. Immune complexes were analyzed by immunoblotting with the indicated antibodies (upper panel). Schematic diagram illustrating ARNT deletion mutants (lower panel).
Mol. Cells 2020;43:935~944 https://doi.org/10.14348/molcells.2020.0122
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