Fig. 6. Promoter optimization in receptor expression with IRES-multicistronic NanoBiT constructs. (A) Cells transfected with EF-driven LgBiT-MYLK2S-IRES-calmodulin-SmBiT and different promoter-driven receptors were treated with ligands, and luciferase activities were measured using a luminometer. V, empty vector. (B) Comparison of the maximum ligand-induced fold-change in luciferase activities depending on receptor expression levels. **P < 0.001 compared to other groups. (C) Basal luciferase activities depending on receptor expression level. **P < 0.001 compared to other groups. RLU, relative light unit. (D) Dose-dependent curves of the maximum ligand-induced luciferase activities in cells expressing UbiC-driven receptors and EF-driven, IRES-linked NanoBiT constructs (compare with Fig. 4D). Also see Supplementary Figs. S7-S9. Ang II, angiotensin II. Data were presented as mean ± SD. All results represent more than three different experiments.

Mol. Cells 2020;43:909~920 https://doi.org/10.14348/molcells.2020.0144

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