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Fig. 5.

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Fig. 5. NanoBiT assay using multicistronic expression vectors. (A) Different basal luciferase activities according to the calmodulin position in the T2A vector system. RLU, relative light unit. (B) Ligand-stimulated luciferase activities in cells expressing 5-HT2AR and NanoBiT constructs of calmodulin and MYLK2S in T2A vectors. Cells were treated with 5-HT and luciferase activities were measured using a luminometer. (C and D) Promoter optimization for IRES-linked NanoBiT constructs. (C) Basal luciferase activities of NanoBiT constructs of calmodulin and MYLK2S linked with IRES in vectors containing different promoters. (D) Cells transfected with different promoter-driven, IRES-linked NanoBiT constructs and UbiC-driven receptor genes were treated with ligands, and luciferase activities were measured using a luminometer. Sub P, substance P; EPI, epinephrine; Ang II, angiotensin II. Data were presented as mean ± SD. All results represent more than three different experiments.
Mol. Cells 2020;43:909~920 https://doi.org/10.14348/molcells.2020.0144
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