Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 4.

Download original image
Fig. 4. Effect of receptor expression levels on ligand-induced luciferase activities. (A) Cells expressing UbiC promoter-driven CM-SmBiT and LgBiT-MYLK2S along with receptors in different promoter constructs were stimulated with ligands, and then the luciferase activities were measured. V, empty vector. (B) Comparison of the maximum ligand-induced fold-change in luciferase activity depending on receptor expression levels. Data were presented as mean ± SD. *P < 0.01, **P < 0.001 compared to other groups. (C) Basal luciferase activities among cells expressing receptors with different expression levels. Data were presented as mean ± SD. **P < 0.001 compared to other groups. RLU, relative light unit. (D) Dose-dependent curves of maximum ligand-induced luciferase activities in cells expressing UbiC-driven receptors. Also see Supplementary Figs. S4-S6. Data were presented as mean ± SD. All results represent more than three different experiments.
Mol. Cells 2020;43:909~920 https://doi.org/10.14348/molcells.2020.0144
© Mol. Cells