Molecules and Cells

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Fig. 3.

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Fig. 3. Different luciferase activation patterns were observed in response to different ligands. (A) HEK293 cells expressing calmodulin-SmBiT and LgBiT-MYLK2S with various membrane receptors were treated with receptor-specific ligands, and luciferase activities were measured in real time using a luminometer. Veh., vehicle; Ang II, angiotensin II; Sub P, substance P; EPI, epinephrine; ISO, isoproterenol; EGF, epidermal growth factor. (B) ERK phosphorylation in response to receptor-specific ligands. Cells expressing the receptors were treated with their cognate ligands for the indicated times and harvested with lysis buffer. Then, 20 μg of cell extracts were analyzed with SDS-PAGE and western blotting using anti-phosphoERK or anti-ERK antibodies. (C) Cells transfected with SRE-Luc and each of the receptor genes were incubated with receptor-specific ligands for 6 h, and then cell lysates were analyzed for luciferase activities. *P < 0.01 compared to no treatment. NT, not treat. Data were presented as mean ± SD. All results represent more than three different experiments.
Mol. Cells 2020;43:909~920
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